涎腺黏液表皮样癌组织SEMA3A与SEMA3B及p53表达临床意义  

Prognostic prediction role of SEMA3A,SEMA3B and p53 proteins in mucoepidermal carcinoma of salivary gland

作　　者：李胜男 查小雨[1] 张杰[1] 陈晓涛[2] 徐江[1] LI Sheng-nan;ZHA Xiao-yu;ZHANG Jie;CHEN Xiao-tao;XU Jiang(Department of Stomatology,Shihezi University Medical School First Affiliated Hospital,Shihezi 832000,P.R.China;Department of Stomatology,People＇s Hospital of Xinj iang Uygur Autonomous,Uygur 830001,P.R.China)

机构地区：[1]石河子大学医学院第一附属医院口腔科,新疆石河子832000 [2]新疆维吾尔自治区人民医院口腔科,新疆乌鲁木齐830001

出　　处：《中华肿瘤防治杂志》2018年第14期997-1002,共6页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(81560442)

摘　　要：目的信号素蛋白SEMA3A和SEMA3B是多种肿瘤的预后决定因素,但在黏液表皮样癌(mucoepidermoid carcinoma,MEC)中研究少见。本研究旨在探讨MEC组织SEMA3A、SEMA3B和p53的表达及与临床病理学特征的关系及其预后意义。方法收集1992-08-19-2016-11-07石河子大学医学院第一附属医院(52例)、新疆维吾尔自治区人民医院(19例)MEC石蜡组织标本71例和石河子大学医学院第一附属医院MEC癌旁正常涎腺组织(normal salivary gland,NSG)石蜡标本35例;采用免疫组化法检测SEMA3A、SEMA3B和p53蛋白表达,χ2检验分析其与临床病理学特征之间的相关性;生存分析采用Kaplan-Meier法,Cox比例风险回归模型分析影响患者无进展生存期(progression-free survival,PFS)的因素。结果MEC组织SEMA3A、SEMA3B和p53阳性表达率分别为33.80%(24/71)、28.17%(20/71)和57.75%(41/71),与NSG组织91.43%(32/35)、85.71%(30/35)和5.71%(2/35)比较,差异均有统计学意义,均P<0.01。MEC组织SEMA3A表达与年龄(χ2=4.589,P=0.032)、淋巴结转移(χ2=4.095,P=0.043)和临床分期(χ2=4.377,P=0.036)有关;SEMA3B表达与性别(χ2=7.848,P=0.005)、年龄(χ2=4.228,P=0.040)、肿瘤大小(χ2=11.146,P=0.001)、淋巴结转移(χ2=6.376,P=0.012)、T分期(χ2=12.138,P=0.007)、N分期(χ2=6.464,P=0.039)和临床分期(χ2=7.360,P=0.007)有关;p53表达与肿瘤大小(χ2=7.832,P=0.005)和临床分期(χ2=8.910,P=0.003)有关。相关性分析显示,MEC组织SEMA3A与p53表达呈负相关(P<0.01,r=-0.474),SEMA3B与p53表达无相关性,P>0.05。Kaplan-Meier单因素生存分析显示,SEMA3A、SEMA3B、p53表达(χ2=6.553,P=0.010;χ2=3.960,P=0.047;χ2=9.448,P=0.002)、性别(χ2=6.502,P=0.011)、淋巴转移(χ2=8.928,P=0.003)、肿瘤大小(χ2=8.524,P=0.004)、T分期(χ2=10.8,P=0.013)、N分期(χ2=11.771,P=0.003)和临床分期(χ2=10.667,P=0.001)是影响患者PFS的相关因素;Cox多因素回归分析显示,性别(HR=0.256,95%CI为0.08~0.813,P=0.021)和T分期(HR=2.051,95%CI为1.006~4.182,P=0.048)可作OBJECTIVE The signal proteins of SEMA3 Aand SEMA3 Bwere critical prognostic factors for multiple cancers,but little was known about in MEC.This study aimed to investigate the relationship between the expression of SEMA3 A,SEMA3 Band p53 in MEC tissues and the clinical pathological features and the corresponding prognosis.METHODS Totally 52 MEC paraffin tissues and 35 normal salivary gland cases（NSG cases,n=35）were collected from the First Affiliated Hospital of Shihezi University and 19 from the People＇s Hospital of Xinjiang Uygur Autonomous Region in 1992-08-19 to 2016-11-07.Immunohistochemical method was used to detect the expression of SEMA3 A,SEMA3 B and p53 proteins.Immunohistochemical method was used to detect the expression of SEMA3 A,SEMA3 Band p53.χ2 test was used to analyze the correlation between the expression of p53 protein and clinicopathological characteristics.Kaplan-Meier was used to analyze the survival of the patients.The Cox proportional risk regression model was used to analyze the factors affecting the progressive survival of the patients.RESULTS The positive expression rates of SEMA3 A,SEMA3 Band p53 proteins in MEC were 33.80%（24/71）,28.17%（20/71）and 57.75%（41/71）respectively,and the differences were all statistically significant compared with NSG,which were 91.43%（32/35）,85.71%（30/35）and 5.71%（2/35）respectively（P〈0.01）.The expression of SEMA3 Aexpression was significantly correlated with age（χ2=4.589,P=0.032）,lymph node metastasis（χ2=4.095,P=0.043）and clinical stage（χ2=4.377,P=0.036）;SEMA3 Bexpression was significantly correlated with sex（χ2=7.848,P=0.005）,age（χ2=4.228,P=0.040）,tumor size（χ2=11.146,P=0.001）,lymph node metastasis（χ2=6.376,P=0.012）,T stages（χ2=12.138,P=0.007）,N stages（χ2=6.464,P=0.039）and clinical stages（χ2=7.360,P=0.007）;p53 expression was significantly correlated with tumor size（χ2=7.832,P=0.005）and clinical stage（χ2=8.910,P=0.003）;There was a negative correlation between SEMA

关 键 词：涎腺黏液表皮样癌 SEMA3A SEMA3B P53 免疫组织化学 预后 

分 类 号：R739.87[医药卫生—肿瘤]