响应面法优化芡茎多糖脱蛋白工艺  

Optimization of Deproteinized Process from Euryale Petioles and Pedicel Polysaccharide by Response Surface Methodology

作　　者：王倩 吴啟南[1,2] 许一鸣 戴仕林[1] 乔晶晶[1] 薛敏 黄晓燕 WANG Qian;WU Qi-nan;XU Yi-ming;DAI Shi-lin;QIAO Jing-jing;XUE Min;HUANG Xiao-yan(College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing 210023,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省中药资源产业化过程协同创新中心,江苏南京210023

出　　处：《食品工业科技》2018年第18期149-155,共7页Science and Technology of Food Industry

基　　金：国家"十二五"科技支撑计划项目(2011BAI04B06);江苏省中药资源产业化过程协同创新中心(2011);江苏省高校中药学优势学科建设工程项目

摘　　要：目的:研究芡茎多糖脱蛋白最优工艺。方法:以芡茎多糖蛋白脱除率和多糖损失率为指标,考察沙维积(Sevag)法、三氯乙酸(trichloroacetic acid,TCA)法、木瓜蛋白酶法、木瓜蛋白酶-TCA法4种方法对芡茎多糖脱蛋白的效果,从而筛选得木瓜蛋白酶-TCA法为较优方法。利用响应面法对木瓜蛋白酶法脱蛋白工艺进行优化,并将最优工艺与TCA法结合处理1次。结果:木瓜蛋白酶-TCA法最优工艺为加酶量580 U·m L-1,酶解温度30℃,酶解时间2.0 h,p H7.0,TCA体积分数8.0%,在此条件下,芡茎多糖蛋白脱除率为85.1%,多糖损失率为16.3%。结论:木瓜蛋白酶-TCA法是一种较优的芡茎多糖脱蛋白方法。Objective： To optimize deproteinized technology of polysaccharide from Euryale petioles and pedicel. Methods： With the rates of deproteination and polysaccharide loss rate as indexes,four deproteination methods,including Sevag,trichloroacetic acid（ TCA）,papain and papain-TCA（ papain hydrolysis followed by TCA precipitation） were compared in our study,the response surface methodology was performed with a Box-Behnken central compostite experiment to optimize deproteinization conditions,and the optimal process was treated with TCA once. Results： The optimal conditions were as follows： hydrolyzing substrates with papain dosage of 580 U·mL^-1,hydrolysis temperature of 30 ℃,hydrolysis time of 2.0 h,pH of 7.0,the concentration of TCA 8.0%. Under this condition,the deproteinated rate was up to 85.1%,and the rate of polysaccharide loss rate was 16.3%. Conclusion： The papain-TCA may become a great candidate for Euryale petioles and pedicel polysaccharides purification.

关 键 词：芡 多糖 脱蛋白 木瓜蛋白酶-TCA法 响应面分析法 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]