云南丽江和吉林靖宇11种越橘果实花色苷组分的分析  

Analysis of Anthocyanins Components of 11 Blueberries in Yunnan Lijiang and Jilin Jingyu

作　　者：臧慧明 吴林[1,2] 徐德冰 王雪松 张强[3] ZANG Hui-ming;WU Lin;XU De-bing;WANG Xue-song;ZHANG Qiang(College of Horticulture,Jilin Agricultural University,Changchun 130118,China;Jilin Province Pulan High-Tech Co.,Ltd,Changchun 130000,China;Institute of Biological Engineering,Beijing Polytechnic,Beijing 100176,China)

机构地区：[1]吉林农业大学园艺学院,吉林长春130118 [2]吉林省普蓝高科技有限公司,吉林长春130000 [3]北京电子科技职业学院生物工程学院,北京100176

出　　处：《食品工业科技》2018年第18期209-213,217,共6页Science and Technology of Food Industry

基　　金：国家星火项目(2015GA660008);农业科技成果转化项目(2014GB2B100021);吉林省中青年科技创新领军人才及团队项目(20160519014JH);吉林省省级粮食生产发展专项资金(科研育种项目)(2014-33)

摘　　要：采用高效液相色谱法对云南丽江、吉林靖宇11种越橘果实花色苷组分进行测定。通过标准品的分析,建立了飞燕草素-3-半乳糖苷、飞燕草素-3-葡萄糖苷、矢车菊素-3-半乳糖苷、矢车菊素-3-葡萄糖苷、锦葵色素-3-半乳糖苷、锦葵色素-3-葡萄糖苷的回归方程,相关系数为0.9939~0.9968,精密度RSD为2.00%~3.73%,回收率在98.90%~100.99%,方法准确可行。通过6种标准品的对比,供试的15个样品中,所有越橘品种均有飞燕草素-3-半乳糖苷、矢车菊素-3-半乳糖苷、锦葵色素-3-半乳糖苷3种花色苷,飞燕草素-3-半乳糖苷平均含量为223.99μg/g、锦葵色素-3-半乳糖苷平均含量为153.34μg/g,二者占总花色苷的65%。采用欧氏距离聚类分析表明,集群1为飞燕草素-3-半乳糖苷含量较高的三种越橘,代表品种为丽江雷戈西、丽江奥尼尔、靖宇杜克;集群2为6种花色苷总含量较高的越橘品种,代表品种为丽江北陆与靖宇早蓝,说明飞燕草素-3-半乳糖苷含量与花色苷总含量是评价越橘花色苷特点的重要因子。The anthocyanins components of 11 blueberries in Yunnan Lijiang and Jilin Jingyu were determined by HPLC. The regression equation of the concentration of delphinidin-3-galactoside,delphinidin-3-glucoside,cyanidin-3-galactoside,cyanidin-3-glucoside,malvidin-3-galactoside and malvidin-3-glucoside were achieved by analysis of standard. The results showed that accuracy and feasibility of the correlation coefficients was 0.9939 - 0.9968,precision RSDs of 2.00% - 3.73%（ n = 5） and the recovery rates of six anthocyanins were in the range of 98.90% - 100.99%. Delphinidin-3-galactoside,cyaniding-3-galactoside,malvidin-3-galactoside were contained in all samples by the intercomparison of 6 standards in 15 samples.The average content of delphinidin-3-galactoside and malvidin-3-galactoside were 223.99 and 153.34 μg/g with65% of the total anthocyanins. Cluster 1 showed the higher contents of delphinidin-3-galactoside was contained by Lijiang Legacy,Lijiang O＇neal,Jingyu Duke and cluster 2 showed the higher content of total anthocyanins of Lijiang Northland,Jingyu Earliblue by the cluster analysis of euclidean distance. In addition,the contents of delphinidin-3-galactoside and total anthocyanins were the evaluated important factor of blueberries anthocyanins characteristics.

关 键 词：越橘 高效液相色谱法 花色苷 聚类分析 

分 类 号：TS207.3[轻工技术与工程—食品科学]