巯基化γ-聚谷氨酸微纳米纤维及其抗氧化性能  

Thiol functionalized poly γ-(glutamic acid) micro/nano fibers and the antioxidant performance

作　　者：徐婷婷 王瑞 李莎[1] 迟波[1] 徐虹[1] XU Tingting;WANG Rui;LI Sha;CHI Bo;XU Hong(State Key Laboratory of Materials.Oriented Chemical Engineering,College of Food Science and Light Industry,Nanjing Tech University,Nanjing 211800,China)

机构地区：[1]南京工业大学食品与轻工学院材料化学工程国家重点实验室,江苏南京211800

出　　处：《生物加工过程》2018年第5期80-84,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(31771049);江苏省先进生物制造创新中心项目(XTB1804)

摘　　要：以L-半胱氨酸(L-Cys)巯基化的γ-聚谷氨酸(γ-PGA-SH)为原料,利用静电纺丝法制备γ-PGA-SH微纳米纤维,经过二硫键(S—S)键交联γ-PGA-SS纤维,并研究交联后γ-PGA-SS纤维抗氧化性能。结果表明:通过核磁共振氢谱、红外光谱分析,发现巯基化聚谷氨酸制备成功,且接枝率为55.6%;扫描电子显微镜(SEM)分析发现,交联后γ-PGA-SS纤维直径由交联前γ-PGA-SH纤维直径的800 nm增加到1 100 nm;热重分析结果显示,交联后的γ-PGA-SS纤维热稳定性有所提高;通过γ-PGA-SS的抗氧化性能研究发现,2 mg的γ-PGA-SS纤维还原能力为0.81(吸光度值),·OH清除率为62.19%,DPPH·清除率为85.84%。综上所述,γ-PGA-SH原料制备成功,并且交联后γ-PGA-SS微纳米纤维有很好的抗氧化性能。-cysteine （L-Cys） was functionalized onto the side of poly γ-（ glutamic acid） （ γ-PGA）chains and then thiol grafting γ-PGA （ γ-PGA-SH） was electrospun for fabricating micro / nano fibersscaffolds.This scaffold can be crosslinked by disulfide bond （ S-S） among the fibers.The antioxidationproperty of γ-PGA-SS fibers scaffolds was studied for tissue engineering application- The results showedγ-PGA-SH polymer was successfully synthesized and the substitution degree was 55- 6% thatcharacterized by proton nuclear magnetic resonance （ 1H NMR） and FT-IR- The diameter of thecrosslinking γ-PGA-SS fibers increased from 800 nm to 1 100 nm compared to uncrosslinking γ-PGA-SH fibers- The thermal stability of crosslinking γ-PGA-SS fibers showed higher than uncrossslinking.Theantioxidation of the γ-PGA-SS fibers showed favorable for 0- 81 （ OD） of reducing power, 62- 19%clearance rate of hydroxyl free radical,85- 84% clearance rate of the DPPH free radical- In conclusion,γ-PGA-SH conjugates were synthesized and the crosslinking γ-PGA-SS fibers werefabricated- Meanwhile,the crosslinking γ-PGA-SS fibers has excellent oxidation resistance.

关 键 词：巯基化 聚谷氨酸 微纳米纤维 抗氧化 生物功能材料 

分 类 号：R362[医药卫生—病理学]