pAKT在恩度诱导Her-2过表达乳腺癌细胞凋亡中的作用  

The role of pAKT in the apoptosis of Her-2 over-expressed breast cancer cell induced by Endostar

作　　者：张世军 李里[2] 金英华[2] 申维喜[2] 姜秋颖[2] 韩楠楠 于静[2] 高雨歌 Zhang Shijun;Li Li;Jin Yinghua;Shen Weixi;Jiang Qiuying;Han Nannan;Yu Jing;Gao Yuge(Department of Oncology,The General Hospital of Dongfeng Aj：filiated to Hubei Medical University,Hubei Shiyan 442001,China;Department of Medical Oneology,The Second Affiliated Hospital of Harbin Medical University,Heilongjiang Harbin 150086,China.)

机构地区：[1]湖北医药学院附属东风医院肿瘤科,湖北十堰442001 [2]哈尔滨医科大学附属第二医院肿瘤内科,黑龙江哈尔滨150086

出　　处：《现代肿瘤医学》2018年第19期3030-3032,共3页Journal of Modern Oncology

基　　金：哈尔滨市应用技术研究与开发项目(编号:2013RFXYJ069)

摘　　要：目的:检测经不同浓度的恩度处理后Her-2过表达乳腺癌细胞MDA-MB-453中pAKT的表达量,初步探讨pAKT在恩度诱导的肿瘤细胞凋亡中的作用。方法:常规体外培养MDA-MB-453细胞,分别用浓度为0μg/ml、200μg/ml、400μg/ml、800μg/ml的恩度处理细胞24 h,Western Blot法检测各浓度恩度处理后细胞的pAKT表达量。结果:随恩度浓度增加,细胞中pAKT表达量逐渐下降。与0μg/ml恩度组相比,200μg/ml组与之无统计学差异(P=0.306),400μg/ml和800μg/ml组与之比较均有统计学差异(P<0.05),但400μg/ml组与800μg/ml组之间无统计学差异(P=0.91)。结论:pAKT表达下调可能参与介导恩度诱导的Her-2过表达乳腺癌细胞凋亡。Objective ：To elicit the role of pAKT placed in the apoptosis of Her-2 over-expressed breast cancer cells induced by Endostar. Methods：Cultivate MDA - MB -453 cells in vitro,and treat each group with Endostar for 24 h,then quantify pAKT expressed in cells of each group by Western Blot method. Results：The expression of pAKT in cells descended with the ascending concentration of Endostar. There was no significant difference between 0μg/ml group and 200 ixg/ml group （P = 0. 306 ）. But pAKT was statistically reduced in 400 μg/ml group and 800 μg/ml group （ P 〈 0.05 ） ,while the difference between these two groups was tiny （ P = 0.91 ）. Conclusion ： Down - regulated of pAKT may be one of those mechanisms uderlying the apoptosis of Her - 2 over - expressed breast cancer cells induced by Endostar.

关 键 词：PAKT 凋亡 HER-2过表达 恩度 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]