Pseudomonas helleri噬菌体PrH-181的分离及生物特性分析  

Isolation and biological characterization of Pseudomonas helleri bacteriophages PrH-181

作　　者：楚乐乐 王宏勋 曾驰[1] 胡申才[1] CHU Le-le;WAN Hong-xun;ZENG Chi;HU Shen-cail(School of Biological and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023;Hubei Province Fresh Food Engineering Research Center,Wuhan 430023)

机构地区：[1]武汉轻工大学生物制药工程学院,武汉430023 [2]湖北省生鲜食品工程技术研究中心,武汉430023

出　　处：《食品科技》2018年第9期7-12,共6页Food Science and Technology

基　　金：国家自然科学基金面上项目(31570175)

摘　　要：Pseudomonas helleri为水产品冷藏过程中的优势腐败菌。为筛选得到能裂解Pseudomonas helleri的烈性噬菌体,采用双层平板法,从采集自不同省份的70个污水和土壤样品中,分离纯化得到了1株裂解活性较强的噬菌体PrH-181。形态学和生化特征实验结果显示,该噬菌体壳体为无尾二十面体结构,对氯仿不敏感,且核酸类型鉴定为RNA。根据ICTV对病毒的分类标准,PrH-181可鉴定为光滑病毒科噬菌体。该噬菌体最佳感染复数(MOI)为0.01,一步生长曲线显示其潜伏期为20 min,爆发期为180 min,裂解量为584。该噬菌体的耐热性良好;最佳生长pH为6.0~7.0,在pH6.0~9.0时其存活率可高达94.1%~98.8%,耐碱力较耐酸力强。Pseudomonas helleri is dominant spoilage organism of aquatic products in the process of refrigeration. This study was isolated and purified a virulent phage, PrH-181 against Pseudomonas helleri from 70 sewage samples and soil samples from different provinces by double-plate method. The results of morphological and biochemical characteristics showed that the PrH-181 is icosahedral structure, not sensitive to chloroform, and the type of its nucleic acid is RNA. According to ICTV（International committee for taxonomy of viruses） classification for viruses, PrH-181 belongs to the Leviviridae family. The optimal MOI（Multiplicity of infection） of PrH-181 is 0.01. The one-step growth curve of PrH-181 showed that the latent period 20 min, the burst period 180 min, and the average burst size is 584 phageparticles/infected cell. The thermal stability of PrH-181 is poor. Its survival rate is 94.1%～98.8% in the range of pH 6.0～9.0 and its optimal pH is 6.0～7.0. PrH-181＇s alkaline tolerance is better than acidic tolerance.

关 键 词：PSEUDOMONAS helleri 噬菌体 分离纯化 生物学特性 核酸鉴定 

分 类 号：TS201.3[轻工技术与工程—食品科学]