重组酶聚合酶扩增技术的研究进展  

Research progress of recombinase polymerase amplification

作　　者：郭正洋 刘钟栋[1] 刘小青[2] 陈晶[2] 兰全学[2] GUO Zheng-yang;LIU Zhong-dong;LIU Xiao-qing;CHEN Jing;LAN Quan-xue(Henan University of Technology,Zhengzhou 450001;Shenzhen Academy of Metrology ＆ Quality Inspection,Shenzhen 518131)

机构地区：[1]河南工业大学,郑州450001 [2]深圳市计量质量检测研究院,深圳518131

出　　处：《食品科技》2018年第9期55-59,共5页Food Science and Technology

基　　金：国家重点研发计划项目(2016YFD0400800);深圳市科技计划项目(JSGG20160606144217004)

摘　　要：重组酶聚合酶扩增技术(Recombinase polymerase amplification,RPA)是一种新型等温扩增技术,它具有操作简单、特异性强、灵敏度高、检测时间短等优点,并且可以在非实验室的条件下完成核酸扩增。目前,RPA技术已经被应用于病毒、致病菌、转基因等领域的检测。通过对不同核酸扩增技术的对比,对RPA技术的扩增原理,RPA扩增产物的检测方法以及RPA技术应用研究作一简单综述,旨在为RPA技术的推广提供参考。Recombinase polymerase amplification（RPA） is a new kind of isothermal amplification technology. It has the advantages of simple operation, strong specificity, high sensitivity, short detection time, and can complete nucleic acid amplification in non-laboratory conditions. Currently, RPA technology has been applied in the detection of viruses, pathogens and transgenic fields. In this review, we compare the different nucleic acid amplification techniques,explain the principle of RPA technology, detection method and application research,aiming at providing some guidance for the promotion of RPA.

关 键 词：重组酶聚合酶扩增 等温 应用 检测方法 

分 类 号：TS201.1[轻工技术与工程—食品科学]