Effects of Ligustrazine on Airway Inflammation in A Mouse Model of Neutrophilic Asthma  

作　　者：LIU Xiao-ming1 WANG Yong-bin2 WU Qian2 BIAN Zhong-rui2 CHE Xiao-wen2 LIU Xiao-ming;WANG Yong-bin;WU Qian;BIAN Zhong-rui;CHE Xiao-wen

机构地区：[1]Department of Geriatric Medicine, the Affiliated Hospital of Shandong University of Traditional Chinese Medicine [2]Department of Respiratory Medicine, the Second Hospital of Shandong University

出　　处：《Chinese Journal of Integrative Medicine》2018年第5期353-358,共6页中国结合医学杂志（英文版）

基　　金：Supported by the Key Scientific and Technological Project of Shandong Province(No.2014GSF119012);China

摘　　要：Objective: To investigate the effects of ligustrazine(LTZ) on airway inflammation in a mouse model of neutrophilic asthma(NA). Methods: Forty healthy C57 BL/6 female mice were randomly divided into 4 groups using a random number table, including the normal control, NA, LTZ and dexamethasone(DXM) groups, with 10 rats in each group. The NA mice model was established by the method of ovalbumin combined with lipopolysaccharide sensitization. At 0.5 h before each challenge, LTZ and DXM groups were intraperitoneally injected with LTZ(80 mg/kg) or DXM(0.5 mg/kg) for 14 d, respectively, while the other two groups were given the equal volume of normal saline. After last challenge for 24 h, the aerosol inhalation of methacholine was performed and the airway reactivity was measured. The bronchoalveolar lavage fluid(BALF) was collected. The Wright-Giemsa staining was used for total white blood cells and differential counts. The levels of cytokines interleukin(IL)-17 and IL-10 were detected by enzyme-linked immunosorbent assay. The pathological change of lung tissue was observed by hematoxylin eosin staining. Results: The airway responsiveness of the NA group was significantly higher than the normal control group(P<0.05), while those in the LTZ and DXM groups were significantly lower than the NA group(P<0.05). The neutrophil and eosinophil counts in the LTZ and DXM groups were significantly lower than the NA group(P<0.05), and those in the LTZ group were significantly lower than the DXM group(P<0.05). There were a large number of peribronchiolar and perivascular inflammatory cells in filtration in the NA group. The airway inflammation in the LTZ and DXM groups were significantly alleviated than the NA group. The infiltration in the LTZ group was significantly reduced than the DXM group. Compared with the normal control group, the IL-17 level in BALF was significantly increased and the IL-10 level in BALF was significantly decreased in the NA group(P<0.05). LTZ and DXM treatment significantly decreased IL-17 levels and increasObjective: To investigate the effects of ligustrazine(LTZ) on airway inflammation in a mouse model of neutrophilic asthma(NA). Methods: Forty healthy C57 BL/6 female mice were randomly divided into 4 groups using a random number table, including the normal control, NA, LTZ and dexamethasone(DXM) groups, with 10 rats in each group. The NA mice model was established by the method of ovalbumin combined with lipopolysaccharide sensitization. At 0.5 h before each challenge, LTZ and DXM groups were intraperitoneally injected with LTZ(80 mg/kg) or DXM(0.5 mg/kg) for 14 d, respectively, while the other two groups were given the equal volume of normal saline. After last challenge for 24 h, the aerosol inhalation of methacholine was performed and the airway reactivity was measured. The bronchoalveolar lavage fluid(BALF) was collected. The Wright-Giemsa staining was used for total white blood cells and differential counts. The levels of cytokines interleukin(IL)-17 and IL-10 were detected by enzyme-linked immunosorbent assay. The pathological change of lung tissue was observed by hematoxylin eosin staining. Results: The airway responsiveness of the NA group was significantly higher than the normal control group(P<0.05), while those in the LTZ and DXM groups were significantly lower than the NA group(P<0.05). The neutrophil and eosinophil counts in the LTZ and DXM groups were significantly lower than the NA group(P<0.05), and those in the LTZ group were significantly lower than the DXM group(P<0.05). There were a large number of peribronchiolar and perivascular inflammatory cells in filtration in the NA group. The airway inflammation in the LTZ and DXM groups were significantly alleviated than the NA group. The infiltration in the LTZ group was significantly reduced than the DXM group. Compared with the normal control group, the IL-17 level in BALF was significantly increased and the IL-10 level in BALF was significantly decreased in the NA group(P<0.05). LTZ and DXM treatment significantly decreased IL-17 levels and increas

关 键 词：LIGUSTRAZINE neutrophilic asthma airway inflammation CYTOKINE 

分 类 号：R285.5[医药卫生—中药学]