黑粒小麦资源的籽粒淀粉组分和SGP-1蛋白组成研究  

Study on Compositions of Grain Starch and SGP-1 Protein in Black Grain Wheat

作　　者：孟雅宁[1,2] 王恰恰 张业伦[1] 兰素缺[1] 李光威[1] 李杏普[1] 古东月 MENG Ya-ning;WANG Qia-qia;ZHANG Ye-lun;LAN Su-que;LI Guang-wei;LI Xing-pu;GU Dong-yue(Institute of Cereal and Oil Crops of Hebei Academy of Agriculture and Forestry Sciences,Hebei Research Station of Crop Gene Resource ＆ Germplasm Enhancement,Ministry of Agriculture.P.R.China,Hebei Labo-ratory of Crop Genetics and Breeding,Shijiazhuang 050035,China;Institute of Cash Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,China;Guantao County Agricultural and Animal Husbandry Bureau,Guantao 057750,China)

机构地区：[1]河北省农林科学院粮油作物研究所农业部河北作物基因资源与种质创制科学观测实验站河北省作物遗传育种实验室,河北石家庄050035 [2]河北省农林科学院经济作物研究所,河北石家庄050051 [3]馆陶县农牧局,河北馆陶057750

出　　处：《河北农业科学》2018年第4期56-59,共4页Journal of Hebei Agricultural Sciences

基　　金：国家科技支撑项目(2013BAD01B02-11);国家自然科学基金项目(31201209);国家重点研发项目(2016YFD0100102-5);河北省科技支撑项目(16226320D)

摘　　要：针对目前世界上缺乏高直链淀粉和高抗性淀粉小麦基因资源的问题,采用SDS-PAGE电泳检测等方法,对冀紫439等43份黑粒小麦资源的籽粒淀粉组分和SGP-1 (SGP-A1、SGP-B1、SGP-D1)蛋白组成进行了鉴定。结果共检测到缺失SGP-A1的材料有11份,未发现SGP-B1和SGP-D1缺失材料;直链淀粉占淀粉总量超过30%的材料有7份;选择12份材料测定抗性淀粉含量,其中,高抗性淀粉含量的材料有5份。以上结果为小麦高抗性淀粉育种奠定了基础。In view of the lack of wheat genetic resources with high amylose and high resistant starch in the present world,by using the method of SDS-PAGE electrophoresis detection,the grain starch components and SGP-1 （SGP-A1、SGP-B1 and SGP-D1） protein of 43 accessions of black wheat genetic resources such as Jizi 439 were identified. The results showed that 11 accessions were lack of SGP-A1. We did not find the materials lacking SGP-B1 and SGP-D1. Seven accessions were identified with amylose contents more than 30% of the total starch. The resistant starch content of 12 accessions selected were determined,and among them 5 accessions with high resistant starch contents were identified. The above results laid a foundation for wheat breeding of high resistant starch.

关 键 词：小麦 SGP-A1 直链淀粉 支链淀粉 

分 类 号：S512.1[农业科学—作物学]