转基因甜菜H7-1品系LAMP现场快速检测方法的建立  

Establishment of LAMP rapid field detection method for genetically modified sugar beet H7-1

作　　者：谌鸿超[1] 蒋静[1] 郑江[1] 樊彦莉 张舒亚[1] 杨捷琳[1] CHEN Hong-chao;JIANG Jing;ZHENG Jiang;FAN Yan-li;ZHANG Shu-ya;YANG Jie-lin(Shanghai Entry-Exit Inspection and Quarantine Bureau,Shanghai 200135,China)

机构地区：[1]上海出入境检验检疫局,上海200135

出　　处：《上海农业学报》2018年第5期63-67,共5页Acta Agriculturae Shanghai

基　　金：上海市科委项目(12DZ0503103)

摘　　要：建立了转基因甜菜H7-1品系特异性基因片段检测的LAMP方法,并对该方法进行了特异性、灵敏性、稳定性测试以及实际样品的应用测试。结果表明:该方法具有特异性,能检出质量分数0. 5%的转基因甜菜H7-1品系,灵敏度高,稳定性好,简便快速,可用于现场检测。A LAMP method for detecting specific gene fragments of genetically modified sugar beet H7-1 was established ,and the specificity, sensitivity, stability and practical application of the method were tested. The results showed that the method was specific and could detect 0.5% （mass fraction） genetically modified sugar beet H7-1 with high sensitivity and good stability. The method was simple and rapid, and could be used for field detection.

关 键 词：转基因甜菜 LAMP 检测方法 

分 类 号：S566.3[农业科学—作物学]