α-细辛脑长循环脂质体冻干工艺的研究  

Study on freeze-drying process of the long-circulated lyophilized liposome of α-asarone

作　　者：陈颖[1] 伍善广 叶娟[1] 陈艳婷[1] CHEN Ying;WU Shanguang;YE Juan;CHEN Yanting(Guangdong Food and Drug Vocational College,Guangzhou 510520,China;Guangxi University of Science and Technology,Liuzhou 545006,China)

机构地区：[1]广东食品药品职业学院实验实训中心,广东广州510520 [2]广西科技大学医学院,广西柳州545006

出　　处：《广西科技大学学报》2018年第4期54-58,共5页Journal of Guangxi University of Science and Technology

基　　金：2015年度广东省医学科研基金项目(A2015260)资助

摘　　要：采用乙醇注入法制备α-细辛脑长循环脂质体,以冻干后的外观和再分散性作为筛选冻干保护剂的评价指标,确定以蔗糖和甘露醇联用作为冻干保护剂,两者的配比为糖∶醇=3∶7,总含量为20%.以冻干率为评价指标,通过正交试验对冻干工艺参数进行优化,确定冻干曲线中预冻温度为-50℃,预冻时间为9 h;升温时间为9 h;主升华干燥温度为-30℃,维持12 h,压力为15 Pa;之后以5℃/h的速度升温;解析干燥温度为35℃,维持5 h,压力为10 Pa.制备的α-细辛脑长循环脂质体冻干工艺重复性、稳定性好,生产可行性高.α-asarone liposomes were prepared by ethanol injection. The appearance and redispersibility of freezedried α-asarone liposomes were selected as evaluation indexes for lyophilization protectant, the combination of saccharose and mannitol was used as a freeze-drying protective agent. The cryoprotective was 20% saccharose- mannitol （3 ： 7） and the optimum process of freeze-drying was ： pre-freeze for 9 h at -50℃, the heating-up time was 9 h; primary-drying for 12 h at -30℃, vacuum was 15 Pa; the temperature of desorption was 35℃ and maintaining 5 h, vacuum was 10 Pa. Stable α-asarone long-circulated lyophilized liposome could be obtained by screening cryoprotectives and optimizing freeze-drying process parameters and the physicochemical characters were good. The confirmatory test has proofed the feasibility and stability of the process.

关 键 词：真空冷冻干燥 Α-细辛脑 长循环前体脂质体 正交试验 冻干工艺 

分 类 号：TQ460.7[化学工程—制药化工]