温州市单核细胞增生李斯特菌的毒力基因及血清学分型和分子分型研究  

Study of serotyping, virulence genes and molecular typing of Listeria monocytogenes isolates in Wenzhou, China

作　　者：章乐怡[1] 林梅芬 李毅[1] 胡玉琴[1] 洪程基[1] 谢爱蓉[1] 梅玲玲[2] 张云怡[2] ZHANG Leyi;LIN Meifen;LI Yi;HU Yuqin;HONG Chengji;XIE Airong;MEI Lingling;ZHANG Yunyi(Wenzhou Center for Disease Control and Prevention,Zhejiang Wenzhou 325000,China;Zhejiang Provincial Center for Disease Control and Prevention,Zhejiang Hangzhou 310051,China)

机构地区：[1]温州市疾病预防控制中心,浙江温州325000 [2]浙江省疾病预防控制中心,浙江杭州310051

出　　处：《中国食品卫生杂志》2018年第5期468-472,共5页Chinese Journal of Food Hygiene

基　　金：国家重点研发计划(2017YFC1601503);浙江省医药卫生科技计划(2018KY034)

摘　　要：目的了解温州市近十年单核细胞增生李斯特菌分离株的血清型、毒力基因及分子分型特征。方法用聚合酶链式反应(PCR)方法对单核细胞增生李斯特菌进行血清型及毒力基因检测;用多位点序列分型(MLST)方法对单核细胞增生李斯特菌进行分子分型,并绘制MLST数据的最小生成树。结果 97株单核细胞增生李斯特菌分离株分为4种血清型,以血清型1/2b、1/2a为优势血清型,占比分别为48.45%(47/97)、35.05%(34/97);而毒力基因iap、prfA基因阳性率均为100.00%(97/97),hlyA、inlA基因阳性率均为97.94%(95/97),plcB基因阳性率为96.91%(94/97)。其中患者分离株5种毒力基因阳性率均为100.00%(6/6)。97株单核细胞增生李斯特菌分离株得到20个MLST型别,其中ST87型是优势型别,其次为ST121和ST9,ST1和ST779型是患者特有的,ST2、ST3、ST5型分布于食品和患者分离株。结论温州市不同来源的单核细胞增生李斯特菌分离株分子型别呈多态性,食品和患者分离株存在相同的ST型,且这些菌株大部分携带毒力基因,具有潜在的致病性,因此食品中单核细胞增生李斯特菌污染的潜在风险不容忽视。Objective To determine the main serogroups, virulence genes and molecular typing of Listeria monocytogenes isolates in Wenzhou, and investigate clustering patterns and pathogenicity of the local strains. Methods Polymerase chain reaction（PCR） was applied to determine the serogroups and virulence genes of the isolates. Multilocus sequence typing（MLST） was used to identify molecular types of the strains. Results Ninety-seven strains were divided into four serogroups. The major serogroups were 1/2 b（48.45%,47/97） and 1/2 a（35.05%,34/97）. The positive rate of pathogenicity gene iap and prfA were both 100.00%（97/97）. All strains from patients carried five virulence genes,the overall hemolysin gene hlyA and inlA were 97.94%（95/97） and plcB was 96.91%（94/97）. These ninety-seven stains were divided into twenty MLST types including the prevalent ST87, ST121 and ST9, patient-specific ST1 and ST779 as well as ST2, ST3 and ST5 that were found both in food and in patients. Conclusion This result demonstrated a high prevalence and genetic polymorphism of Listeria monocytogenes from diverse sources in Wenzhou. The majority of isolates carried pathogenicity-related genes, which posed a potential threat to the public health. The surveillance and management should be strengthened to prevent the infectious listeriosis.

关 键 词：单核细胞增生李斯特菌 多位点序列分型 分子分型 血清学分型 毒力基因 食源性致病菌 温州 

分 类 号：R155[医药卫生—营养与食品卫生学]