产胞外多糖能力不同的混合乳酸菌对二型糖尿病的影响  

Effects of Mixed Lactic Acid Bacteria Which Producted Exopolysaccharide on Type 2 Diabetic Mice

作　　者：李向菲[1] 周炜 印伯星 房东升 王刚[1] 赵建新[1] 张灏[1] 陈卫[1] Li Xiangfei;Zhou Wei;Yin Boxing;Fang Dongsheng;Wang Gang;Zhao Jianxin;Zhang Hao;Chen Wei(School of Food Science and Technology,Jiangnan University,Wuxi 214122,Jiangsu;Kangyuan Dairy Co.,Ltd.,Yangzhou University,Yangzhou 225004,Jiangsu)

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]扬州市扬大康源乳业有限公司,江苏扬州225004

出　　处：《中国食品学报》2018年第9期16-24,共9页Journal of Chinese Institute Of Food Science and Technology

摘　　要：本文研究产胞外多糖能力不同的乳酸菌组合对二型糖尿病的影响。通过苯酚-硫酸法测定乳酸菌的胞外多糖产量,按照产胞外多糖能力大小将15株乳酸菌分为高产多糖组、中产多糖组和低产多糖组。通过建立二型糖尿病小鼠模型,探讨这3组胞外多糖产量不同的乳酸菌组合的降血糖效果,最终发现相比于低产多糖组,高产多糖组和中产多糖组具有较好的降低禁食和餐后血糖,改善葡萄糖耐受和胰岛素抵抗的作用;而中产多糖组又在对糖基化血红蛋白、胰岛素和炎症反应的影响上优于高产多糖组和低产多糖组。抗氧化和血脂研究表明,这3个混菌组合都能在一定程度上发挥类似的改善作用。筛选中产多糖组乳酸菌作为缓解糖尿病的潜在降血糖制剂,这将为后续的研究提供理论依据。In this study, the antidiabetic effect of Lactobacillus which produced different concentration of exopolysaccharide is explored on high-fat and low-dose streptozotocin induced type 2 diabetes in mice. The exopolysaccharide levels of lactic acid bacteria were determined using phenol-sulfuric acid method. According to the exopolysaccharide production capacity, the fifteen strains were divided into high exopolysaccharide group, middle exopolysaccharide group and low exopolysaccharide group. Oral administration of high and middle exopolysaccharide strains could more efficiently reduce fasting and postprandial blood glucose levels, improve glucose tolerance and insulin resistance than that in the low exopolysaccharide group in the type 2 diabetic mice. However, the diabetic related indexes such as glycosylated hemoglobin, insulin and inflammatory response, were better regulated by middle exopolysaccharide group compared to that in the high and low exopolysaccharide group. In addition, the three groups could play a similar improving role in the antioxidant and serum lipid at a certain extent. Therefore, the strains in the middle exopolysaccharide group could be as potential probiotics to ameliorate diabetes, and these results provided a strong theoretical basis for the further studies.

关 键 词：口服糖耐量 胰岛素抵抗 抗氧化 血脂 

分 类 号：TS201.4[轻工技术与工程—食品科学]