酿酒酵母戊糖转运蛋白及C6/C5共代谢菌株的研究进展  

Progress in research of pentose transporters and C6/C5 co-metabolic strains in Saccharomyces cerevisiae

作　　者：汪城墙[1,2] 李洪兴[3,2] 徐丽丽 沈煜[2] 侯进 鲍晓明[3,2] Chengqiang Wang2,3, Hongxing Li1,3, Lili Xu1, Yu Shen3, Jin Hou3, and Xiaoming Bao1,3(1 Shandong Provincial Key Lab of Microbial Engineering, School of Bioengineering, Qilu University of Technology, Jinan 250353, Shandong, China; 2 Shandong Key Laboratory of Agricultural Microbiology, College of Life Sciences, Shandong Agricultural University, Tai＇an 271018, Shandong, China; 3 The State Key laboratory of Microbial Technology, College of Life Sciences, Shandong University, Jinan 250100, Shandong, China)

机构地区：[1]山东农业大学生命科学学院山东省农业微生物重点实验室,山东泰安271018 [2]山东大学生命学院微生物技术国家重点实验室,山东济南250100 [3]齐鲁工业大学生物工程学院山东省微生物工程重点实验室,山东济南250353

出　　处：《生物工程学报》2018年第10期1543-1555,共13页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(Nos.31870063,31470219,31470166,31700094);山东省自然科学基金(No.ZR2014CL003);山东省科技重大专项(新兴产业)(No.2015ZDXX0403B02)资助

摘　　要：充分利用木质纤维素中的糖分是提高以此类生物质为原料生产二代燃料乙醇经济盈利性的基本要求,也是实现其他生物基化学品规模化生产的基础。传统的乙醇生产微生物酿酒酵母Saccharomyces cerevisiae具有独特的生产性能及内在优势,是备受关注的底盘细胞,但其不能有效地利用戊糖。利用代谢工程、合成生物学策略,对二代燃料乙醇生产专用酿酒酵母的精准构制持续研究了30余年,已明显改善了其对木糖/葡萄糖的乙醇共发酵能力。近年来关注点集中在早期忽略的限速步骤即糖转运环节的研究上,以期实现不同糖分各行其道、高效专一性转运蛋白各行其责的二代燃料乙醇生产特种酿酒酵母所需的糖转运理想状态。文中主要综述了酿酒酵母戊糖转运蛋白的研究进展,及酿酒酵母的木糖和L-阿拉伯糖代谢工程的研究现状。One of the requirements for increasing the economic profitability on the large-scale production ofsecond-generation ethanol and other bio-chemicals using lignocellulose biomass as raw materials is efficient hexose and pentose utilization. Saccharomyces cerevisiae, the traditional ethanol producer, is an attractive chassis cell due to its robustness towards harsh environmental conditions and inherent advantages. But S. cerevisiae cannot utilize pentose. The precision construction of suitable strains for second-generation bio-ethanol production has been taken for more than three decades based on the principle of metabolic engineering and synthetic biology. The resulting strains have improved significantly co-fermentation of glucose and xylose. Recently, much attentions have been focused on sugar transport, which is one of the limiting but formerly ignored step for ethanol production from both glucose and xylose, to get the desired state that different sugars could efficiently delivered by their individual specific transporters. In this paper, the progress on sugar transporters of S. cerevisiae was reviewed, and the research status of xylose and/or L-arabinose metabolic engineering in S. cerevisiae were also presented.

关 键 词：糖转运蛋白 戊糖 代谢工程 纤维素乙醇 芽殖酵母 

分 类 号：TS261.11[轻工技术与工程—发酵工程]