牡丹花蕊多糖三相分离纯化及其理化性质  

Study on the purification of polysaccharide from Peony Stamen by three phase partitioning and physicochemical property

作　　者：罗磊[1] 薛依涵 杨永庆 朱文学[1] LUO Lei1, XUE Yi-han1, YANG Yong-qing2, ZHU Wen-xue1(1. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang , Henan 471023, China ; 2. Luoyang Peony Biological Science and Technology Research Institute, Luoyang, Henan 471023, China)

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471023 [2]洛阳牡丹生物科技研究院,河南洛阳471023

出　　处：《食品与机械》2018年第8期123-128,134,共7页Food and Machinery

基　　金：河南省科技攻关项目(编号:182102110140);河南省高校科技创新团队计划(编号:17IRTSTHN0l6)

摘　　要：采用三相分离法纯化牡丹花蕊多糖,并对其理化性质进行分析。结果表明,纯化的最优条件是pH 7、硫酸铵质量分数10%、叔丁醇与提取液体积比为2. 0,该条件下多糖回收率为69%,蛋白质去除率为73%,纯化后多糖纯度为99. 24%,色素清除率为95. 36%; PMP柱前衍生化高效液相色谱(PMP-HPLC)分析表明,牡丹花蕊多糖的单糖组成为鼠里糖、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖,其摩尔比为1. 24:1. 59:2. 00:9. 11:1. 68;傅立叶红外变换光谱(Fourier transform infrared spectroscopy,FR-IR)与氢核磁共振波谱(~1H nuclear magnetic resonance,1H NMR)结果表明,牡丹花蕊多糖是一种以半乳吡喃糖为主的酸性多糖,并且同时存在α-构型和β-构型,以β-构型为主。Three phase partitioning was used to purify the polysaccharide from peony stamen and its physicochemical properties were analyzed. The optimal conditions of purification were obtained as follows： pH 7,ammonium sulfate concentration（w/v） 10% and ratio of t-butyl alcohol/crude extract（v/v）. Under this condition,the polysaccharide recovery was 69%and the protein removal rate was 73%. The purity rate of the purified polysaccharide was 99. 24%,and 95. 36% pigment was removed by three phase partitioning. The Rresult of high performance liquid chromatography（HPLC） analysis showed that it was composed of rhamnose,galacturonic acid,glucose,galactose and arabinose,and the molar ratio was 1. 24：1.59：2. 00 ：9. 11：1. 68. The results of the Fourier transform infrared spectroscopy（FR-IR） and1 H nuclear magnetic resonance（-1H NMR）showed that the polysaccharide from peony stamen was an acidic polysaccharide mainly composed of galacto-pyranose,and there are both α-and β-configuration,in which β-configuration accounted for a large proportion.

关 键 词：牡丹花蕊 多糖 三相分离法 理化性质 

分 类 号：TS201.2[轻工技术与工程—食品科学]