小儿呋麻滴鼻液微生物限度检查方法适用性研究  

Applicability Study on Microbial Limit Test for Ephedrine and Furacillin Nasal Drops for Infants

作　　者：田怀平[1] 林志燕[1] 王冉冉[1] 王玲[1] 杜毅[1] Tian Huaiping;Lin Zhiyan;Wang Ranran;Wang Ling;Du Yi(Xinhua Hospital Affiliated to Shanghai Jiaotong University,School of Medicine,Shanghai 200092,China)

机构地区：[1]上海交通大学医学院附属新华医院,上海200092

出　　处：《儿科药学杂志》2018年第11期45-48,共4页Journal of Pediatric Pharmacy

摘　　要：目的:建立小儿呋麻滴鼻液的微生物限度检查方法。方法:参照《中国药典》2015年版的方法,制备1∶10供试液,薄膜过滤法进行样品前处理,分别进行微生物计数检查和控制菌检查的方法适用性实验。结果:薄膜过滤法可有效消除小儿呋麻滴鼻液供试品的抗菌活性。微生物计数检查的5种实验菌的回收比值均在0. 5～2. 0之间。控制菌检查中铜绿假单胞菌、金黄色葡萄球菌和大肠埃希菌都能够检出。结论:采用1∶10稀释法和薄膜过滤法的检查方法和检验条件,方法适用性实验已确认可以进行小儿呋麻滴鼻液的微生物限度检查。Objective： To establish a method for microbial limit test on ephedrine and furacillin nasal drops for infants. Methods： By reference to Chinese Pharmacopoeia（ 2015 Edition）,the 1 ∶ 10 test solution was prepared,and the sample pretreatment was carried out under membrane-filter procedure,the applicability experiments of microbial count test and control bacteria test were conducted respectively. Results： The membrane-filter procedure could effectively eliminate the antibacterial active of ephedrine and furacillin nasal drops. The recovery ratios of the five experimental bacteria examined by microbial count were within 0. 5 ～ 2. 0. And seudomonas aeruginosa,staphylococcus aureus and escherichia coli can be detected in control bacteria. Conclusion： With the inspection method and test conditions of 1 ∶ 10 dilution method and membrane-filter procedure,the applicability experiments has confirmed that microbial limit test of ephedrine and furacillin nasal drops for infants can be performed.

关 键 词：小儿呋麻滴鼻液 微生物限度检查 微生物计数检查 控制菌检查 薄膜过滤法 

分 类 号：R926[医药卫生—药学]