西藏灵菇来源酵母菌产谷胱甘肽能力比较及提取条件优化  

The Yeasts from Tibetan Kefir:Comparison of Glutathione Production and Extraction Optimization

作　　者：孟利 何静 刘峰 朱莹 张迷 李秀凉 Meng Li;He Jing;Liu Feng;Zhu Ying;Zhang Mi;Li Xiuliang(College of Life Science Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education,Key Laboratory of Microbiology,Heilongjiang University,Harbin 150080;Heilongjiang Academy of Agricultural Sciences,Safety and Quality Institute of Agricultural Products,Heilongjiang Academy of Agricultural Science,Harbin 150086)

机构地区：[1]黑龙江大学农业微生物技术教育部工程研究中心微生物重点实验室,哈尔滨150080 [2]黑龙江省农业科学院农产品质量安全研究所,哈尔滨150086

出　　处：《中国农学通报》2018年第24期77-81,共5页Chinese Agricultural Science Bulletin

基　　金：哈尔滨市科技创新人才研究专项资金项目"西藏灵菇调节2型糖尿病小鼠菌群关键菌株研究"(2015RQQXJ042)

摘　　要：为获得高产谷胱甘肽菌株,以16株西藏灵菇来源酵母菌为研究对象,采用DTNB法测定酵母菌体提取液中的GSH含量,并以Y15为出发菌株,用单因素试验及正交试验优化提取谷胱甘肽的条件。结果表明,菌株Y19、Y15谷胱甘肽产量较高。采用Y15菌体冻融后热水浸提,料液比为1:6,p H 2,沸水浴50 min,其提取量最大,为49.90 mg/L。Y15产谷胱甘肽较高,其谷胱甘肽提取条件为菌株Y15的利用提供了数据支持。To obtain the strains with high glutathione production, 16 yeasts from Tibetan Kefir were used as materials, GSH content in the yeast extract was detected with DTNB method. Taking Y15 as the original yeast,glutathione extraction condition was optimized by single factor experiment and the orthogonal test. The resultshowed that, yeast Y19 and Y15 had higher glutathione production. The optimized condition of glutathioneextracted from Y15 was freeze-thaw hot water extraction, solid-liquid ratio 1：6, p H 2, and boiling water bathfor 50 minutes. The yield of glutathione was 49.90 mg/L. Y15 is a high glutathione producing yeast, theglutathione extraction condition provides data basis for Y15 further utilization.

关 键 词：酵母菌 谷胱甘肽 筛选 提取 正交试验 

分 类 号：TS202.3[轻工技术与工程—食品科学]