植物乳杆菌野生质粒分析及其提取方法的优化  

Analysis of Native Plasmid of Lactobacillus plantarum and Optimization of Its Extraction Methods

作　　者：萨初拉[1] 苏少锋[1] 吴青海[1] 其布日[1] 高娃[1] 王蕴华[1] 刘红葵[1] 呼和[1] Sachula;SU Shao-feng;WU Qing-hai;Qiburi;Gaowa;WANG Yun-hua;LI U Hong-kui;Huhe(Research Center of Biotechnology,lnner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)

机构地区：[1]内蒙古农牧业科学院生物技术研究中心,内蒙古呼和浩特010031

出　　处：《畜牧与饲料科学》2018年第11期6-9,共4页Animal Husbandry and Feed Science

基　　金：国家自然科学基金项目(31260566)

摘　　要：乳酸菌质粒提取研究具有很长的历史。目前已有多个有效的乳酸菌质粒提取方法被报道,其中多数以乳酸球菌为研究对象,而植物乳杆菌质粒提取有效方法报道较少。该研究以植物乳杆菌为研究对象,通过对比分析并优化已报道的乳酸菌质粒提取方法,得到了高效且适于平台期植物乳杆菌野生质粒的提取方法。通过该方法进一步分析了植物乳杆菌野生质粒数和高拷贝质粒,为后续构建植物乳杆菌表达系统所需供体质粒和受体菌株提供了重要依据。Studies on plasmid extraction of lactic acid bacteria have a long history. Up to date, a number of effective plasmid extraction methods of lactic acid bacteria have been reported. Most of them focused on Lactococcus, however, information on plasmid preparation technique of Lactobacillus plantarum is scare. In this study, the extracting efficiency of different plasmid preparation methods in Lactobacillus plantarum were comparatively investigated, and the reported plasmid extraction methods were optimized. A high-efficient native plasmid preparation method for Lactobacillus plantarum in plateau phase was developed. In addition, the number of native plasmid and high-copy-number plasmid of Lactobacillus plantarum were assessed, which provided technical references for the preparation of donor plasmid and the selection of receptor required in further construction of Lactobacillus plantarum expression system.

关 键 词：植物乳杆菌 质粒 提取 优化 

分 类 号：Q939.117[生物学—微生物学] TS201.3[轻工技术与工程—食品科学]