血红素加氧酶1对人脊髓星形胶质细胞缺氧/复氧损伤的保护作用  

Protective effect of HO-1 overexpression against hypoxia-reoxygenation injury of human astrocytes of spinal cord

作　　者：梁飞[1] 魏魏[1] 药泽蓉[1] 李晓泽[1] Liang Fei;Wei Wei;Yao Zerong;Li Xiaoze(Genetic Laboratory,Changzhi Maternal and Child Health Hospital,Changzhi 046000,China)

机构地区：[1]长治市妇幼保健院医学遗传室,046000

出　　处：《中华临床实验室管理电子杂志》2018年第4期222-225,共4页Chinese Journal of Clinical Laboratory Management(Electronic Edition）

基　　金：山西省卫生厅科研课题项目(201202001)

摘　　要：目的探讨血红素加氧酶1(heme oxygenase, HO-1)过表达对人脊髓星形胶质细胞(humanastrocytesofspinalcord,HA-sp)缺氧/复氧损伤的保护作用。方法通过给予HA-sp氯化钴(250μl/L CoCl_2)刺激模拟缺氧,然后再去除CoCl_2刺激,建立细胞缺氧/复氧损伤模型。采用免疫印迹(westernblot, WB)方法,从蛋白水平上检测不同刺激条件下HA-sp中HO-1的表达。采用四甲基偶氮唑蓝(methylthiazolyldiphenyl-tetrazolium bromide, MTT)方法,分析单纯给予缺氧/复氧刺激后以及上调HO-1表达的同时给予缺氧/复氧刺激后HA-sp的存活率变化。结果缺氧/复氧刺激条件下,HA-sp中HO-1蛋白过表达。利用锌原卟啉(ZnPPIX)抑制缺氧/复氧刺激条件下HO-1过表达后,HA-sp在缺氧/复氧刺激条件下存活率明显降低(P<0.01);与单纯给予缺氧/复氧刺激后HA-sp的存活率相比,用钴原卟啉(CoPP)上调HA-sp中HO-1表达的同时给予缺氧/复氧刺激,细胞存活率明显升高(P<0.01)。结论利用CoPP上调HA-sp中HO-1的表达,具有抗细胞缺氧/复氧损伤的保护作用。ObjectiveTo investigate the protective effect of overexpressed heme oxygenase-1 （HO-1） in hypoxia-reoxygenation of human astrocytes of spinal cord（HA-sp）. MethodsHypoxia-reoxygenation mode was established by oxygen provision after CoCl2 stimulation. The expression of HO-1 under different stimulation was detected by Western blotting. MTT assay was used to examine the relative survival of cell under hypoxia-reoxygenation as well as after up-regulated HO-1. ResultsHO-1 was upregulated by hypoxia/re-oxygen stimulation. The relative survival rate of HA-sp under hypoxia-reoxygenation was signiflcantly decreased after HO-1 protein overexpression was inhibited by ZnPPIX （P〈 0.01）, while the relative survival rate of HA-sp which was pretreated by CoPP was signiflcantly increased compared with that of cells under hypoxia-reoxygenation （P〈0.01）. ConclusionHO-1 overexpression by CoPP exerts protective effect on hypoxia-reoxygenation injury of HA-sp. The result of this study may offer new thought for the therapy of hypoxia-reoxygenation injury of human spinal cord in the future.

关 键 词：血红素加氧酶1 人脊髓星形胶质细胞 缺氧/复氧 存活率 

分 类 号：R741[医药卫生—神经病学与精神病学]