梨汁饮品中有机磷农药的分散液液微萃取条件的优化  

Optimization of Dispersive Liquid-liquid Microextraction for Organophosphorus Pesticide Residues in Pear Juice Beverage

作　　者：陶昆[1] 张建群[1] 申丽静[1] 曹巧巧[1] 张俐勤[1] TAO Kun;ZHANG Jianqun;SHEN Lijing;CAO Qiaoqiao;ZHANG Liqin(Branch of Agriculture and Environment,Jiaxing Vocational and Technical College,Jiaxing 314036)

机构地区：[1]嘉兴职业技术学院农业与环境分院,嘉兴314036

出　　处：《食品工业》2018年第11期162-166,共5页The Food Industry

基　　金：2016年度浙江省嘉兴市公益性应用技术研究计划(2016AY23022)

摘　　要：建立了分散液液微萃取(以下简称DLLME)方法与气相色谱法联用快速检测梨汁饮料中9种有机磷农药残留量的分析方法。选用四氯乙烯作为萃取剂,丙酮作为分散剂进行DLLME操作,采用FPD检测器进行分析。采用Central Composite Design (CCD)响应面法对分散液液微萃取条件进行了优化,得到优化条件为:萃取时间2.85 min、四氯乙烯用量49.23μL、丙酮用量0.50 mL。结果表明:当加标质量浓度为0.10、1.0和2.0μg/L时, 9种有机磷农药的平均加标回收率达到88.5%～121.0%,相对标准偏差均小于6%,取得了令人满意的试验结果。A gas chromatograpy（GC） method for the quick determination of nine organophosphorous pesticides in pear juice beverage was developed by using dispersive liquid-liquid microextraction（DLLME） as sample preparation methods.Tetrachloroethylene was selected as an extractant and acetone used as a dispersant for DLLME operation coupled with flame photometric detector（FPD）. Some experimental parameters influencing the extraction efficiency, such as the types and volume of the dispersants and extractants, were optimized by response surface methodology combined with central composite design（CCD）. The optimal conditions were obtained as follow： 2.85 min of extraction time 49.23 μL of extractant 0.50 mL of dispersant. When the spiked concentrations were 0.10, 1.0 and 2.0 μg/L, the results showed that the recovery values of nine organophosphorous pesticides in fruit juice beverage were range from 88.5% to 121.0% and the relative standard deviations was under 6%. With the advantages of quickness, low toxicity and high enrichment factors, the proposed method was applicable for the analysis of organophosphorus pesticides in pear juice beverage.

关 键 词：分散液液微萃取(DLLME) 响应面法 有机磷 农药残留 梨汁饮品 

分 类 号：O658.2[理学—分析化学] TS255.44[理学—化学]