miRNA在牛乳过敏小鼠模型中的表达及嗜酸乳杆菌的干预作用  

The Abnormal Expression of miRNA in β-lg Sensitized Mouse Model and Intervention Effect of Lactobacillus acidophilus

作　　者：孙伊乔 李艾黎[1] 张超 李爽 李巍巍 Sun Yiqiao;Li Aili;Zhang Chao;Li Shuang;Li Weiwei(Key Laboratory of Dairy Science,Ministry of Education,College of Food Science,Northeast Agriculture University,Harbin 150030)

机构地区：[1]乳品科学教育部重点实验室东北农业大学食品学院,哈尔滨150030

出　　处：《中国食品学报》2018年第10期16-23,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：黑龙江省教育厅骨干教师基金资助项目(1254G008)

摘　　要：观察β-乳球蛋白(β-Lg)过敏小鼠模型中miRNA的异常表达和Th17相关细胞因子分泌的相关性,同时研究嗜酸乳杆菌的干预作用,探究益生菌缓解牛乳过敏的分子机制。BALB/c小鼠随机被分为正常对照组,β-Lg过敏模型组和低、中、高剂量嗜酸乳杆菌干预组,以ELISA法检测过敏小鼠血清中Th17相关细胞因子的分泌,RT-PCR法检测过敏小鼠脾脏组织CD4+T细胞中与炎症相关的miRNA基因表达,并对两者进行相关性分析。在此基础上研究嗜酸乳杆菌干预过敏模型对miRNA表达的影响。与正常对照组比较,过敏组小鼠血清IgE,ILI7,IL-6,TNF-α及IL-21水平显著增高(P<0.05);其脾脏组织CD4+T细胞中miR-155,miR-21,miR-9和miR-146a mRNA表达量明显上调(P<0.05),与Th17细胞的RORγt及IL-17mRNA表达水平呈正相关(P<0.05)。嗜酸乳杆菌干预可显著下调过敏小鼠Th17细胞亢进,以及上述miRNA基因的异常表达(P<0.05),具有剂量依赖性。以上结果显示,miRNA在β-Lg过敏中发挥重要作用,嗜酸乳杆菌缓解过敏的作用可能与其调节miRNA表达密切相关。The present study was planned to analyze the correlations between Th17-related cytokine production and abnormal miRNA expression in allergic mouse model, and investigate the intervention effect of L. acidophilus in this process. BALB/c mice were randomly divided into normal control group, β-Lg allergy group, and L. acidophilus group（low, medium high dose）. After the allergen challenge, the levels of serum Th17-related cytokine were measured by ELISA, the miRNA expression in spleen tissue CD4＋T cells were measured by real-time PCR, and made the correlation analysis. Furthermore,the effect of L. acidophilus on the miRNA expression were analyzed. The results showed that sensitization with β-lg led to develop the allergic response, the serum IgE, IL-17, IL-6, TNF-α and IL-21 expression were increased in β-lg-sensitized mice compared to the controls（P〈 0. 05）. In addition, the miR-155, miR-21, miR-9 and miR-146 a mRNA expression of β-lg-sensitized mice were positively correlated with IL-17 and RORγt mRNA（ P〈 0. 05）. Compared to allergic mice, oral administration of L. acidophilus suppressed hypersensitivity responses, decreased the expression of IL-17 and RORγt mRNA, together with inhibited abnormal miRNA expression in a dose-dependent manner. These findings suggested that L. acidophils could regulate miRNA expression, which may be responsible for the suppression of allergic symptoms.

关 键 词：嗜酸乳杆菌 牛乳过敏 MIRNA TH17细胞 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]