鸢尾异黄酮的分离纯化及抑菌活性研究  

Extraction,Purification and Antibacterial Activity Evaluation of Isoflavones from Iris Tectorum Maxim

作　　者：赵华龙[1] 毛涵[1,2] 王远平 王露 汤新慧[1] ZHAO Hua-long;MAO Han;WANG Yuan-ping;WANG Lu;TANG Xin-hui(Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection,Yancheng Teachers University,Yancheng 224051,China;College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 210009,China)

机构地区：[1]盐城师范学院江苏省滩涂生物资源与环境保护重点建设实验室,江苏盐城224051 [2]南京工业大学生物与制药工程学院,江苏南京210009

出　　处：《食品与药品》2018年第6期418-422,共5页Food and Drug

基　　金：江苏省"青蓝工程"科技创新团队项目

摘　　要：目的研究鸢尾中异黄酮的分离纯化工艺及抑菌活性。方法醇提法提取野生鸢尾叶中的异黄酮,采用硅胶柱层析结合半制备高效液相色谱法对鸢尾异黄酮进行分离纯化和含量测定,以乙酸乙酯:丙酮:无水乙醇=5:1:0.5(v/v/v)为柱层析洗脱剂对鸢尾异黄酮进行分离纯化;以甲醇:0.4%磷酸=90:10(v/v)为高效液相色谱(HPLC)流动相,柱温30℃,检测波长265 nm,进样量20μl,流速1 ml/min测定含量和纯度。采用牛津杯法检测鸢尾异黄酮对枯草芽孢杆菌和大肠杆菌的抑菌活性。结果与结论分离纯化后的鸢尾异黄酮纯度为91.29%,提取率为1.62%。抑菌试验结果表明,鸢尾异黄酮对大肠杆菌和枯草芽孢杆菌均有显著的抑制作用,最小抑菌浓度分别为1.25 mg/ml和0.625 mg/ml,表明鸢尾异黄酮有显著抑菌活性。Objective To study the separation and purifcation process and antimicrobial activity of iso？avones in Iris tectorum Maxim. Methods Total isoflavones were extracted from leaves of wild Iris tectorum Maxim by alcohol. Silica gel column chromatography combined with semi-preparative high performance liquid chromatography （HPLC） were used for purifcation and content determination of iso？avones respectively. Ethyl acetate：acetone：ethanol=5：1：0.5 （v/v/v） were used as column chromatography eluent. Methanol：0.4 % phosphoric acid=90：10 （v/v） was used as mobile phase for HPLC. The column temperature was 30 ℃, the detection wavelength was 265 nm, the sample size was 20 μl, and the ？ow rate was 1 ml/min. The antibacterial activity of the iso？avones against E. coli and B. subtilis were detected by the Oxford cup method. Results and Conclusion The purity of the iso？avones after purifcation was 91.29 % and the extraction rate was 1.62 %. The results of bacteriostatic test showed that the iso？avones from Iris tectorum Maxim inhibited E. coli and B. subtilis signifcantly, with the minimum inhibitory concentration of 1.25 and 0.625 mg/ml, respectively, suggesting that iso？avones from Iris tectorum Maxim has signifcant antibacterial activity.

关 键 词：柱层析 鸢尾异黄酮 分离纯化 高效液相色谱 抑菌活性 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]