藏药五味甘露药浴颗粒对AA模型大鼠的药效作用研究  

Pharmacodynamics of Tibetan medicated-bath Wuwei-Ganlu-Yaoyu-Keli on AA rats

作　　者：王静 张艺[2] 杨花[2] 央嘎 王天虹[2] WANG Jing;ZHANG Yi;YANG Hua;YANG Ga;WANG Tianhong(School of management Chengdu University of TCM,Chengdu 611137;School of National Medicine Chengdu University of TCM,Chengdu 611137,China;Tibetan Traditional Medical College,Lhasa 850000,China)

机构地区：[1]成都中医药大学管理学院,四川成都611137 [2]成都中医药大学民族医药学院,四川成都611137 [3]西藏藏医学院,西藏拉萨850000

出　　处：《中国民族民间医药》2018年第18期12-16,共5页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：国家地区科学基金项目(81260672)

摘　　要：目的:观察藏药五味甘露药浴颗粒对AA模型大鼠的药效作用。方法:除正常组大鼠外,采用弗氏完全佐剂造模,随机分为模型组、地塞米松组(0. 150 mg/kg)、五味甘露药浴颗粒高剂量组(11. 80 g/L)、中剂量组(5. 90 g/L)、低剂量组(2. 95 g/L),药浴组40℃药水浴30 min/d,模型组和正常组40℃温水浴30 min/d。给药四周,采用致炎后足容积测量、测定大鼠胸腺、脾脏脏器系数、HE染色法观察致炎侧足踝关节滑膜病理改变,评价藏药五味甘露药浴颗粒对AA大鼠的治疗效果。结果:与模型组比较,五味甘露药浴颗粒高剂量组、中剂量组、低剂量组AA大鼠原发性足肿胀度均有减轻,滑膜细胞增生、炎细胞浸润、关节软骨破坏均有不同程度的改善。结论:经藏药浴五味甘露药浴颗粒治疗后,原发性足肿胀有明显下降的趋势,踝关节病理形态有所改善,大鼠的胸腺、脾脏系数有升高的趋势,提示该药可以明显减轻关节炎症程度,增强机体的免疫功能,对类风湿关节具有抗炎消肿的治疗作用。Objective To observe the efficacy of Tibetan medicine-bath Wuwei-Ganlu-Yaoyu-Keli（ WGYK） on AA rats.Methods Except for the control group,the rat model was made by Freund＇s complete adjuvant. The rats were randomly divided into adjuvant arthritis group（ AA）,dexamethasone acetate group（ DMA,0. 150 mg/Kg）,high dose Wuwei-Ganlu-Yaoyu-Keli group（ WGYKh,11. 80 G/L）,moderate dose Wuwei-Ganlu-Yaoyu-Keli group（ WGYKm,5. 90 G/L）,and low dose Wuwei-Ganlu-Yaoyu-Keli group（ WGYKl,2. 95 G/L）. In the Tibetan medicine-bath group,the bath temperature was 40 ℃ and bathed 30 min/d. The control group and the AA model group used 40 ℃ warm water and and bathed 30 min/d too. After four weeks,by Measured of phlogistic foot volume and the coefficient of the thymus and spleen organs of rats,and used HE staining to observe the pathological changes of the synovium of the ankle joints on the inflammatory side,we evaluated the therapeutic effect of Tibetan medicine-bath on AA rats. Results Compared with the AA model group,the primary foot swelling degree of the WGYKh group,the WGYKm group and the WGYKl group was reduced,and the synovial cell proliferation,the inflammatory cell infiltration and the articular cartilage damage were improved to some extent in different degrees. Conclusion After the treatment of the Tibetan medicine-bath WGYK,the primary foot swelling was obviously decreased,the pathological morphology of the ankle joint was improved,the thymus and spleen coefficient of the rats increased,suggesting that the drug could obviously reduce the degree of inflammation of joint and enhance the immune function of the body,and have the effect of anti inflammation and reduce swelling of the rheumatoid joints.

关 键 词：五味甘露药浴颗粒 类风湿性关节炎 AA模型大鼠 药效作用 

分 类 号：R-332[医药卫生]