Dynamic Perception of Jasmonates by the F-Box Protein C0l1  

作　　者：Jianbin Yan Ruifeng Yao Li Chen Suhua Lit Min Gut Fajun Nan Daoxin Xie 

机构地区：[1]MOE Key Laboratory of Bioinformatics, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China [2]State Key Laboratory of Drug Research, the National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai201203, China [3]These authors contributed equally to this article.

出　　处：《Molecular Plant》2018年第10期1237-1247,共11页分子植物（英文版）

基　　金：This research was financially supported by the National Key R＆D Program of China （2016YFA0500500）, the National Natural Science Foundation of China （31630085 and 31421001）, the China Postdoctoral Science Foun-dation （2017T100063 and 2017M610078）, the China Association for Sci-ence and Technology （YESS20160148）, and the Postdoctoral Fellowship of Tsinghua-Peking Joint Center for Life Sciences.

摘　　要：Jasmonates （JAs） are cyclic fatty acid-derived phytohormones that regulate diverse aspects of plant defense and development. The endogenous active JA molecule （＋）-7-iso-JA-L-Ile （JA-Ile） and its analog coronatine trigger formation of a complex with the F-box protein C011 and JAZ repressors to induce degra- dation of the JAZs through the 26S proteasome pathway in a COil-dependent manner. To reveal the forma-tion process of COI1-JA-JAZ ternary complex, we employed several biochemical approaches to examine how JA is dynamically perceived. These analyses showed that the COI1 proteins of Arabidopsis and rice bind JA with appreciable binding affinity and revealed the kinetics and thermodynamics of the COI1-JA-JAZ ternary complex. Our results suggest that COI1 is the primary receptor perceiving the active JA molecule to initially form a COI1-JA complex that subsequently recruits JAZs for further signal transduction.Jasmonates （JAs） are cyclic fatty acid-derived phytohormones that regulate diverse aspects of plant defense and development. The endogenous active JA molecule （＋）-7-iso-JA-L-Ile （JA-Ile） and its analog coronatine trigger formation of a complex with the F-box protein C011 and JAZ repressors to induce degra- dation of the JAZs through the 26S proteasome pathway in a COil-dependent manner. To reveal the forma-tion process of COI1-JA-JAZ ternary complex, we employed several biochemical approaches to examine how JA is dynamically perceived. These analyses showed that the COI1 proteins of Arabidopsis and rice bind JA with appreciable binding affinity and revealed the kinetics and thermodynamics of the COI1-JA-JAZ ternary complex. Our results suggest that COI1 is the primary receptor perceiving the active JA molecule to initially form a COI1-JA complex that subsequently recruits JAZs for further signal transduction.

关 键 词：PHYTOHORMONE RECEPTOR JASMONATE C011 

分 类 号：Q51[生物学—生物化学] X173[环境科学与工程—环境科学]