miR-143/145侧翼序列多态性与膀胱癌遗传易感性研究  

Association between polymorphisms in the flanking region of miR-143/145 and risk of bladder cancer

作　　者：刘孝德[1] 张鹏[1] Liu Xiaode;Zhang Peng(Department of Urology,Qinghai Hospital of Traditional Chinese medicine,Qinghai 810000,China)

机构地区：[1]青海省中医院泌尿外科,青海西宁810000

出　　处：《国际泌尿系统杂志》2018年第6期890-892,共3页International Journal of Urology and Nephrology

摘　　要：目的 探讨miR-143/145侧翼序列rs4705342和rs4705343多态性与膀胱癌遗传易感性的关系.方法 采用基于医院的病例对照研究,收集106例膀胱癌患者和162例对照人群外周静脉血样本,TaqMan探针法和聚合酶链反应-限制性长度片段多态性分析rs4705342和rs4705343多态性.结果 rs4705342位点CC基因型和C等位基因显著降低了膀胱癌的发病风险（CC与TT相比,调整OR=0.30,95％CI：0.10 ～0.88,P=0.018;C与T相比,调整OR=0.60,95％CI：0.40～0.89,P=0.01）.单倍型分析显示,rs4705342C-rs4705343T单倍型显著降低了膀胱癌的发病风险（OR =0.33,95％CI：0.15～0.74）.结论 miR-143/145侧翼序列rs4705342CC基因型可能是膀胱癌发病的保护因素.Objective To explore the association between rs4705342 and rs4705343 polymorphisms in the flanking region of miR-143/145 and risk of bladder cancer.Methods A hospital-based case-control study was performed.Peripheral venous blood samples were taken from 106 patients with bladder cancer and 162 control individuals.The rs4705342 polymorphism was genotyped using TaqMan assay and the rs4705343 polymorphism was genotyped using polymerase chain reaction-restriction fragment length polymorphism.Results The rs4705342CC genotype and C allele were associated with a decreased risk of bladder cancer（CC vs.TT：adjusted OR =0.30,95％ CI：0.10-0.88,P =0.018;C vs.T：adjusted OR =0.60,95 ％ CI：0.40-0.89,P =0.01）.Haplotype analysis showed that rs4705342C-rs4705343T haplotype was associated with a decreased risk of bladder cancer compared with the rs4705342T-rs4705343T haplotype （OR =0.33,95 ％ CI：0.15-0.74）.Conclusions The rs4705342CC genotype in the flanking region of miR-143/145 may protect against the development of bladder cancer.

关 键 词：膀胱肿瘤 微RNAS 疾病遗传易感性 多态现象 遗传 

分 类 号：R737.14[医药卫生—肿瘤]