蜂毒溶血肽(Melittin)cDNA的克隆及序列分析  

The Cloning and Sequencing of cDNA Encoded Melittin

作　　者：李大力[1] 王丹[1] 王关林[2] 方宏筠[2] 汪信[1] 

机构地区：[1]南京理工大学化工学院,江苏南京210094 [2]辽宁师范大学生物工程研究所,辽宁大连116029

出　　处：《淮海工学院学报（自然科学版）》2002年第3期34-37,共4页Journal of Huaihai Institute of Technology:Natural Sciences Edition

基　　金：南京理工大学博士后基金资助项目 (2 0 0 1-0 3 18)

摘　　要：为了克隆得到正确序列的蜂毒溶血肽 c DNA,对蜂毒中的蜂毒溶血肽进行了研究。实验从蜜蜂毒腺中提取总 RNA,通过 RT- PCR方法扩增得到了蜂毒溶血肽的 c DNA,扩增产物克隆到p T7Blu- T载体 ,转化大肠杆菌 JM1 0 9,DNA序列分析结果表明克隆得到的蜂毒溶血肽 cThe melittin in bee poison gland was studied to obtain the correct cDNA encoded melittin. During the experiment, the total RNA was obtained from bee poison gland and cDNA en\|coded melittin was obtained by RT PCR. After that, the obtained product was ligated to pT7Blu T vector and the recombined plasmid was transformed to the E. coli strain of JM109. The result of DNA sequence analysis demonstrated that the sequence of cDNA encoded melittin was correct.

关 键 词：蜂毒溶血肽 CDNA PCR扩增 DNA序列分析 基因克隆 重组质粒 毒素蛋白 

分 类 号：Q785[生物学—分子生物学] Q516