化学物质对不同发育天数海湾扇贝幼虫变态的诱导  被引量：5

Induction of metamorphosis of differentdayold bay scallop Argopecten irradians by chemical cues</em> <p class='article-op'> <span id="bdshare" class="bdshare_t bds_tools get-codes-bdshare baidufx"><span><span class="bds_more" style="display: block; width: 62px; height: 29px; background: url(/images/share.gif) no-repeat!important; padding: 0;"></span></span></span> </p> <p class="article-source"> <a href ='/article/read.aspx?id=6820461' class='btnTitle btnIsView' target='_blank'><img alt='在线阅读' src='/images/btn-read.png'/></a><a class='btnTitle btnIsView' href="javascript:showdown('vrMAfK/CrCSZp1Vhc6GOUMIHIp91i6VMRNxoqZLpfDg=')"><img alt="下载全文" src="/images/btn-down.png" /></a> <input id="hiddowninfo" type="hidden" value="vrMAfK/CrCSZp1Vhc6GOUMIHIp91i6VMRNxoqZLpfDg="/> </p> <div class="collect" style="display: none"> <h5>我的收藏</h5> <div class="cnt"> <p> 名 称：<input type="text" name="collTitle" id="collSumTitle" /> </p> <p> 注 释：<input type="text" name="collRemark" id="collSumRemark" /> 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href='/writer/article.aspx?id=100000008967411' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e4%bd%95%e4%b9%89%e6%9c%9d%5d' title='何义朝[1]'>何义朝[1]</a> <a href='/writer/article.aspx?id=100000006534085' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e5%bc%a0%e7%a6%8f%e7%bb%a5%5d' title='张福绥[1]'>张福绥[1]</a> <em> </em></p><p class='organ'><strong>机构地区：</strong><a href='/organ/article.aspx?id=18929&subid=&showname=&searchtype=' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=S%3d%e4%b8%ad%e5%9b%bd%e7%a7%91%e5%ad%a6%e9%99%a2%e6%b5%b7%e6%b4%8b%e7%a0%94%e7%a9%b6%e6%89%80' title='[1]中国科学院海洋研究所,山东青岛266071'>[1]中国科学院海洋研究所,山东青岛266071</a> <em> </em></p><p class='from'><strong>出　　处：</strong><a href='/journal/summary.aspx?gch=98270X' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=J%3d%5b%e4%b8%ad%e5%9b%bd%e6%b0%b4%e4%ba%a7%e7%a7%91%e5%ad%a6%5d' title='中国水产科学'>《中国水产科学》</a>2002年第3期228-233,共6页<em>Journal of Fishery Sciences of China</em></p><p class='fund'><strong>基　　金：</strong>国家自然科学基金资助项目(39970588).</p><p class='abstrack'><strong>摘　　要：</strong>自海湾扇贝(Argopectenirradians)受精后第10天起,定期取一定量海湾扇贝幼虫,分别置于不同浓度梯度的KCl、肾上腺素、去甲肾上腺素、L-DOPA、5-羟色胺、GABA、茶碱和咖啡因等8种诱导物中,处理时间为8h。实验后第14天取出幼虫观察显示,这8种诱导物对不同发育天数海湾扇贝幼虫的变态有着不同的诱导作用。13.42×10-3和20.13×10-3mol/L的KCl对第12天幼虫的变态有抑制作用,变态提高率为负值;当幼虫发育至第13和14天时,上述两浓度的KCl能够明显诱导幼虫变态,变态率均提高20%以上,而对于第16天的幼虫诱导作用有所减弱,变态提高率有所降低;26.85×10-3mol/L的KCl对第12和13天幼虫的变态均有抑制作用,变态提高率为负值,对第14和16天幼虫的变态却有明显持续的诱导作用,变态率分别提高22.98%和37.5%。神经递质肾上腺素、去甲肾上腺素、L-DOPA、5-羟色胺和GABA的诱导作用规律基本相似,即对第13天海湾扇贝幼虫的变态有明显的抑制作用,变态提高率均为负值,而对第14天幼虫的诱导作用较显著。茶碱和咖啡因作为影响细胞内cAMP的物质,其诱导作用规律与神经递质有所不同,对第13天海湾扇贝幼虫变态的诱导效果最好。<em><Abstrcat> The larval Argopecten irradians individuals were sampled regularly after the eggs were fertilized for 10 d and exposed to solutions of KCl (concentration gradients 13.42, 20.13, 26.85 mmol/L), epinephrine (EPI, 0.01, 0.05, 0.1 mmol/L), norepinephrine ( NE,the same as EPI), L-DOPA (the same as EPI), 5-HT (1.0,10.0, 100.0 μg/L) GABA (the same as 5-HT), theophylline (0.1, 1.0 mmol/L) and caffeine(the same as theophylline), respectively, for 8 h. After 14 d, the larvae were fixed by I2 solution and inspected . The 8 chemical cues have different effects on the larval metamorphosis at different age of days that KCl at 13.42 and 20.13 mmol/L can inhibit the metamorphosis of 12dayold larvae, but significantly increase the metamorphosis of 13and 14dayold larvae with the metamorphosis increment rate> 20%, and the inductive effect reduces on day 16. KCl at 26. 85 mmol/L has inhibitive effect on 12 and 13dayold larvae's metamorphosis but can promote the metamorphosis of 14 and 16 dayold larvae with the metamorphosis increment rates of 22.98% and 37.5%, respectively. The inductive effects of neurotransmitters EPI, NE, LDOPA, 5HT and GABA are similar that they all inhibit the larval metamorphosis on day 13 but increase the larval metamorphosis on day 14. The inductive effects of theophylline and caffeine , which influence intracellular CAMP concentration, are different from neurotransmitters that the optimum inductive effects are obtained on day 13.</em></p><p class='subject'><strong>关 键 词：</strong><a href='/subject/article.aspx?id=1176361' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e5%b9%bc%e8%99%ab%5d' title='幼虫'>幼虫</a> <a href='/subject/article.aspx?id=5479989' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e5%8c%96%e5%ad%a6%e7%89%a9%e8%b4%a8%5d' title='化学物质'>化学物质</a> <a href='/subject/article.aspx?id=6376854' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e6%b5%b7%e6%b9%be%e6%89%87%e8%b4%9d%5d' title='海湾扇贝'>海湾扇贝</a> <a href='/zk/search.aspx?key=K%3d%5b%e9%99%84%e7%9d%80%e5%8f%98%e5%bc%82%5d' title='附着变异'>附着变异</a> <a href='/zk/search.aspx?key=K%3d%5b%e4%ba%ba%e5%b7%a5%e8%af%b1%e5%af%bc%e7%89%a9%5d' title='人工诱导物'>人工诱导物</a> <a href='/subject/article.aspx?id=4708207' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e8%8b%97%e7%a7%8d%e5%9f%b9%e8%82%b2%5d' title='苗种培育'>苗种培育</a> </p><p class='class'><strong>分 类 号：</strong><a href='/zk/search.aspx?key=C%3dS968.313' title='S968.313'>S968.313</a>[<a href = '/domain/article.aspx?id=560' >农业科学—水产养殖</a>] </p> </div> <div id="content_wzxg" name="wzxg" class="article-graph"> <object width="695" height="250" classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" id="FlashID"> <param value="../flash/relation.swf" name="movie"> <param value="high" name="quality"> <param value="opaque" name="wmode"> <param value="6.0.65.0" name="swfversion"> <param value="dataUrl=/ajax/articlerelativegraph.ashx?id=6820461" name="flashVars"> <embed width="695" height="250" src="../flash/relation.swf" type="application/x-shockwave-flash" quality="high" wmode="opaque" flashvars="dataUrl=/ajax/articlerelativegraph.ashx?id=6820461"> 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