视网膜色素上皮DNA蓝光损伤及维生素E的保护作用  

Blue Light Induced DNA Damage of Retinal Pigment Epithelium (RPE) Cells and the Protection of Vitamin E

作　　者：林海燕[1] 张晓明[1] 周吉林[1] 

机构地区：[1]浙江大学医学院基础医学系,浙江杭州310031

出　　处：《科技通报》2002年第5期428-430,共3页Bulletin of Science and Technology

摘　　要：目的　探讨维生素 E对视网膜色素上皮 DNA蓝光损伤的保护作用 .方法　视网膜色素上皮细胞分为 3组 :正常组 (不照光 ) ,实验组 (蓝光照射 40 min) ,维生素 E组 (蓝光照射同时给予维生素 E) .采用火箭电泳的方法对各组视网膜色素上皮 DNA损伤进行分析 .结果　各组火箭电泳尾部 DNA含量分别为 1 8.44% ,54.42 %和 2 2 .1 6% .各组差异有显著性意义 (P<0 .0 1 ) .结论　蓝光能对视网膜色素上皮的 DNA产生损伤 ,维生素 E对损伤能起到一定的保护作用 .Objective To evaluate the protection of vitamin E on blue light induced DNA damage of retinal pigment epithelium (RPE) cells.Method The cultured RPE cells were divided into 3 groups: normal control group (no blue light), experimental group(blue light for 40 minutes) and vitamin E group(blue light+100μM vitamin E). Travigen's comet assay kit and euclid comet assay software were used to assay the DNA damage levels. Results The DNA % in the tail in the above three groups were 18.44%,54.42 and 22.16% respectively ( P <0.01). Tail moments were 8.2, 48.3, and 18.4 respectively ( P <0.01).Conclusion Blue light could induce DNA damage to RPE cells and vitamin E could protect the RPE cells from blue light induced DNA damage.

关 键 词：视网膜色素上皮DNA 蓝光损伤 维生素E 保护作用 火箭电泳 保护机制 抗自由基 

分 类 号：R774.13[医药卫生—眼科]