三尖杉酯碱诱导慢性髓系白血病K-562细胞凋亡的实验研究  

Experimental study of K562 cell apoptosis induced by harringtonine

作　　者：李荣[1] 刘晓力[1] 杜庆锋[1] 田红[1] 宋兰林[1] 张燕[1] 杨艺[1] 周淑芸[1] 

机构地区：[1]第一军医大学南方医院血液科,广东广州510515

出　　处：《第一军医大学学报》2002年第9期788-790,共3页Journal of First Military Medical University

基　　金：广东省自然科学基金(970833)

摘　　要：目的阐明三尖杉酯碱（HT）作用于K562细胞的机制。方法应用细胞形态、DNA凝胶电泳和流式细胞仪等检测,观察HT对K562细胞株的作用方式,进一步用RT-PCR方法研究bcr/abl基因在转录水平的改变。结果0.01～100.00μg/ml浓度HT可诱导K562细胞凋亡,并呈时间和剂量依赖性。随着药物作用时间的延长,bcr/abl基因的转录下调。结论低浓度HT就可通过抑制bcr/abl基因的表达,诱导K562细胞凋亡。Objective To elucidate the mechanism by which harringtonine (HT) induces apoptosis in K562 cell line. Methods By means of cell morphology, DNA gel electrophoresis and flow cytometry, we explored the action of HT on K562 cell line. Further study of the changes in bcrlabl gene expression was conducted using reverse transcriptase (RT)-PCR. Results HT induced apoptosis of K562 cells at the concentrations ranging from 0.01 to 100 (Ag/ml, exhibiting dose- and time-dependent increase in apoptotic ratios of the cells subjected to the treatment courses of 12 to 60 h. RT-PCR showed that bcr/abl gene expression was down-regulated after K562 cells had been treated with 10 u,g/ml HT. Conclusion Low concentration of HT can induce apoptosis in K562 cell line, possibly through the down-regulation of bcr/abl gene expression.

关 键 词：三尖杉酯碱 慢性髓系白血病 细胞系 K-562 CML RT-PCR方法 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]