交链孢酚单甲醚对人淋巴细胞增殖反应的影响  

EFFECT OF AME ON PROLIFERATIVE RESPONSE IN HUMAN LYMPHOCYTES

作　　者：李忠[1] 刘桂亭[1] 钱玉珍[1] 宫亚欧[2] 郑智敏[2] 王秀林[2] 甄应中[2] 

机构地区：[1]河南医科大学病理生理教研室 [2]河南医科大学食管癌研究室

出　　处：《肿瘤防治研究》1991年第3期154-155,共2页Cancer Research on Prevention and Treatment

摘　　要：本实验运用PHA诱导的淋巴细胞增殖~3H—TdR掺入法,对食管癌高发区河南省林县粮食中分离的优势污染菌互隔交链孢霉(亦称链格孢菌)的毒素之一——交链孢酚单甲醚(AME)的免疫抑制作用进行了研究。实验结果表明:AME能明显抑制淋巴细胞对~3H—TdR的摄取,在浓度为25、50、75和100μg/ml,其抑制率分别是76.9％、89.2％、91.3％和94.9％,与对照组相比有显著性意义(P<0.01),且存在一定的剂量—效应关系。结果提示AME可能抑制细胞免疫功能。In this paper, we investigated the suppressive effect of AME (alternariol monomethyl ether) on proliferative response in human lymphocytes by use of PHA-induced lymphocyte proliferation SH-TdR incorporation method. The results showed: AME had a significant inhibitory effect on SH-TdR incorporation into lymphocytes. As the concentration of AME was 25ug/ml, 50ug/ml, 75ug/ml and 100μg/ml, its suppressive ratio was 76.9％, 89.2％, 91.3％ and 94.9％ respectively. The effect of AME was significantly different from control (P<0.01), and had does-dependent fashion (Y=12.13-0.10X,r=-0.927, P<0.05, alone). This result suggests that AME could inhibit cellular immune and affect the AME-induced carcinogenesis.

关 键 词：霉菌毒素 淋巴细胞 增殖反应 实验 

分 类 号：R73－354[医药卫生—肿瘤]