TNFα对培养的脐静脉内皮细胞的损伤作用  

Effect of TNFα on endothelial cells of human umbilical vein in culture

作　　者：董红梅[1] 喻伦银[2] 贾宗智[3] 邓末宏[4] 

机构地区：[1]汕头大学医学院法医学教研室,汕头5150312 [2]武汉大学医学院病理学教研室 [3]山西省长治医学院病理教研室,046000 [4]汕头大学医学院附属一院口腔科,515031

出　　处：《临床与实验病理学杂志》2002年第4期401-403,共3页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的 :探讨肿瘤坏死因子α(TNFα)对脐静脉内皮细胞的损伤作用。方法 :将培养的内皮细胞分为对照组和实验组 ,实验组培养液中含有TNFα(4× 10 5U/L) ;通过普通光镜、扫描电镜、透射电镜观察TNFα对内皮细胞形态和超微结构的影响 ,并运用免疫组化染色来观察TNFα对内皮细胞表达细胞间粘附分子 (ICAM 1)的影响。结果 :(1)光镜、电镜观察结果显示 :实验组内皮细胞拉长 ,由原来的多边形变为成纤维状 ,细胞间距不均且变大 ;微绒毛变短甚至呈泡状 ,个别拉长 ,有些部位细胞膜有缺损以及内质网扩张、线粒体凝聚等改变。并且这些改变有时间依赖性。 (2 )实验组内皮细胞ICAM 1表达明显增强。结论 :TNFα(4× 10 5U/L)Purpose To investigate the effects of TNFα on the endothelial cells. Methods The control group, the medium without TNF, and the medium with TNFα(4×10 5 U/L) were included in the experimental group. Damage effect of TNFα was investigated on the endothelial cells by observing cell morphology, ultrastructural changes and the expression of ICAM 1. Results (1) The cell elongation, endoplastic reticula dilation, mitochondria condensation and microvilli shortening could be observed in experimental group. (2) The enhanced expression of ICAM 1 was also seen in experimental group. Conclusion TNFα can damage the endothelial cells of human umbilical vein.

关 键 词：脐静脉 内皮细胞 TNFΑ 肿瘤坏死因子 胞间粘附分子 

分 类 号：R730.3[医药卫生—肿瘤]