温度对重组大肠杆菌生长及外源蛋白表达的影响  

Effect of Temperature on the Growth of Recombinant E.coli and on the Expression of Recombinant Protein

作　　者：叶姣[1] 陈长华[1] 夏杰[1] 付水林[1] 杨雅琴[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《华东理工大学学报（自然科学版）》2002年第4期364-367,共4页Journal of East China University of Science and Technology

摘　　要：对重组大肠杆菌 (E.coli) BL2 1 (DE3 )发酵生产 rh Cu/ Zn-SOD的诱导温度进行了优化 ,考察了 3 7°C和 3 0°C下重组菌的生长规律及产物表达。在 5 L自控发酵罐中的发酵结果表明 :较低温度时菌体的比生长速率较低 ,菌体活性与质粒稳定性有很大程度的改善 ,外源蛋白稳定性与可溶性也有一定的提高 ;3 0°C诱导时 rh Cu/ Zn-SOD的酶活性最高 ,每毫升发酵液的酶活力为 475 7U,约为 3 7°C诱导时的 2 .The expression of rhCu/Zn SOD in recombinant E.coli BL21(DE3) at different induction temperatures was investigated. The growth of E.coli and the expression of the recombinant protein were studied and compared at 37°C or 30°C .The recombinant E.coli was cultivated in 5L fermentor, results of which showed that at 30°C the specific growth rate was reduced while the viability of recombinant E.coli and plasmid stability were greatly increased. The stability and solubility of the recombinant protein were also improved. The maximum enzyme activity of rhCu/Zn SOD expressed at 30°C was increased to 4 757 U/mL,which was 2.7 times as that at 37°C .

关 键 词：外源蛋白 重组大肠杆菌 超氧化物歧化酶 诱导温度 发酵生产 基因重组 基因表达 酶活性 

分 类 号：TQ925[轻工技术与工程—发酵工程] TQ920.6