The expression and significance of CATSPER1 in human testis and ejaculated spermatozoa  

精子特异性钙离子通道(CATSPER1)蛋白在人类睾丸和射出精子中的表达及其意义

作  者:Hong-Gang Li Ai-Hua Liao Xiao-Fang Ding Hui Zhou Cheng-Liang Xiong 

机构地区:[1]Centre of Reproductive Medicine.Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

出  处:《Asian Journal of Andrology》2006年第A03期301-306,386,共5页亚洲男性学杂志(英文版)

摘  要:Aim:To investigate the distribution of cation channel of sperm 1(CATSPER1)protein and the presence of CATSPER1 mRNA in human testis and ejaculated spermatozoa.The influence of anti-human CATSPER1 antibody upon human sperm motility was used to evaluate the function of human CATSPER1 and to estimate its possible use as a target for immunocontraception.Methods:Human ejaculated sperm from normozoospermic donors(n=12)and liquid nitro- gen frozen human testis were used for the study of mRNA and protein expression of CATSPER1 by reverse transcliption polymerase chain reaction(RT-PCR)and immunohistochemistry,respectively.Spermatozoa from nonnozoospermic donors(n=12)were individually processed using a swim-up procedure and were then incubated with CATSPER 1 antibody at final concentrations of 20,4 and 0.8μg/mL.After 1,2 and 6h incubation,progressive motility and fast progressive motility were measured by means of computer-assisted semen analysis.Results:CATSPER1 transcript was detected in both human testis and each human ejaculated semen sample.CATSPER1 protein expressed in the membrane of spermatid and was localized in the principal piece of the sperm tail.The application of CATSPER1 antibody at all concentrations significantly inhibited both progressive motility and fast progressive motility after 1,2 and 6h incubation,and significant dose-dependent changes were observed.Conclusion:CATSPER1 is meiotically and post-meiotically expressed in human testis tissue.CATSPER1 mRNA in human ejaculated spermatozoa could be a more feasible target for study and infertility screening than testis biopsy.In addition,our results suggest that human CATSPER1 could be a possible target for immunocontraception.(Asian J Androl 2006 May:8:301-306)Aim:To investigate the distribution of cation channel of sperm 1(CATSPER1)protein and the presence of CATSPER1 mRNA in human testis and ejaculated spermatozoa.The influence of anti-human CATSPER1 antibody upon human sperm motility was used to evaluate the function of human CATSPER1 and to estimate its possible use as a target for immunocontraception.Methods:Human ejaculated sperm from normozoospermic donors(n=12)and liquid nitro- gen frozen human testis were used for the study of mRNA and protein expression of CATSPER1 by reverse transcliption polymerase chain reaction(RT-PCR)and immunohistochemistry,respectively.Spermatozoa from nonnozoospermic donors(n=12)were individually processed using a swim-up procedure and were then incubated with CATSPER 1 antibody at final concentrations of 20,4 and 0.8μg/mL.After 1,2 and 6h incubation,progressive motility and fast progressive motility were measured by means of computer-assisted semen analysis.Results:CATSPER1 transcript was detected in both human testis and each human ejaculated semen sample.CATSPER1 protein expressed in the membrane of spermatid and was localized in the principal piece of the sperm tail.The application of CATSPER1 antibody at all concentrations significantly inhibited both progressive motility and fast progressive motility after 1,2 and 6h incubation,and significant dose-dependent changes were observed.Conclusion:CATSPER1 is meiotically and post-meiotically expressed in human testis tissue.CATSPER1 mRNA in human ejaculated spermatozoa could be a more feasible target for study and infertility screening than testis biopsy.In addition,our results suggest that human CATSPER1 could be a possible target for immunocontraception.(Asian J Androl 2006 May:8:301-306)

关 键 词:(?)ation channel of sperm 1 TESTIS SPERMATOZOA MOTILITY IMMUNOCONTRACEPTION HUMAN 

分 类 号:R321[医药卫生—人体解剖和组织胚胎学]

 

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