从黑曲霉发酵的果胶粗酶中分离纯化内切聚半乳糖醛酸酶  

Purification of an endo-polygalacturonase from Aspergillus niger

作　　者：林洪宝[1] 傅蓉[1] 薛伟明[1] 郭春腾[1] 饶平凡[1] 

机构地区：[1]福州大学生物工程研究所,福建福州350002

出　　处：《福州大学学报（自然科学版）》2002年第5期632-635,共4页Journal of Fuzhou University(Natural Science Edition)

摘　　要：使用硫酸铵分级沉淀、CM -SephadexC - 5 0弱阳离子、POROSPE疏水层析和POROSHQ2 0强阴离子交换分离技术 ,从黑曲酶发酵的果胶酶粗品中纯化出一种电泳纯的果胶酶 .经SDS -PAGE电泳测定其相对分子量约 41.8kD ,最适pH和最适温度分别为 5 .0和 36℃ .A pectinase was identified and isolated from a commercial Aspergillus niger pectinase preparation. The crude enzyme preparation, which was prepared by precipitation of the water extract of the culture of Aspergillus niger with ammonium sulfate, was further fractionated by three steps of chromatography of cation exchange, hydrophobic interaction and anion exchange to obtain an electrophoretically homogeneous pectinase. The molecular weight of the purified enzyme was estimated by SDS-PAGE to be 39.4 kD under non-reduced conditions and 41.8 kD under reduced conditions, with the optimum pH at 5.0, and the optimum temperature at 36?℃.

关 键 词：内切聚半乳糖醛酸酶 黑曲霉 果胶酶 分离 纯化 发酵生产 硫酸铵分级沉淀 POROS疏水层析 

分 类 号：TQ925.3[轻工技术与工程—发酵工程]