Endomorphins inhibit contractile responses of rat thoracic aorta rings induced by phenylephrine and angiotensin Ⅱ in vitro^1  

内吗啡肽抑制苯肾上腺素和血管紧张素Ⅱ诱导的离体大鼠胸主动脉环收缩(英文)

作　　者：祁元明[1] 杨顶建[1] 段昕 杨帆[1] 李世荣 沈剑敏 王锐[1] 

机构地区：[1]兰州大学生命科学学院生物化学与分子生物学系,兰州中国730000

出　　处：《Acta Pharmacologica Sinica》2002年第1期42-46,共5页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (No 20072014),and the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions of MOE,China

摘　　要：To study the effects of opioid receptor agonists endomorphin-1 and -2 on contractile responses of rat thoracic aorta rings to phenylephrine ( PE ) and angiotensin Ⅱ(AngⅡ), and their possible mechanism in vitro. METHODS: Isometric tension recording was progressed in thoracic aorta rings from Wistar rats. RESULTS: Pretreatment of morphine, endomorphin-1 and-2 (0.1, 1, and 10 μmol/L) could inhibit the contractile responses of the endothelium-intact aorta rings to PE (0.1 μmol/L) and Ang Ⅱ(1μmol/L) in a concentration-dependent manner ( P 【 0.01), but could not inhibit the contraction of rings without endothelium (P】0.05). Naloxone (1μmol/L) could partially antagonize the effects of endomorphine-1 and -2 ( P 【 0.01). N-nitro-L-arginine (L-NNA, 10μmol/L) or endothelial rubbing could completely blocked the effects of morphine, endomorphine-1 and -2 ( P 【 0.01 ). CONCLUSION: Endomorphin-1 and -2 could inhibit PE- and Ang Ⅱ-induced contractions of rat aorta rings, which was partially by naloxone-sensitive mechanism and related to the release of nitric oxide from vascular endothelium.目的:研究吗啡、内吗啡肽-1和内吗啡肽-2对由苯肾上腺素(PE)和血管紧张素(Ang)Ⅱ诱导离体大鼠胸主动脉环收缩的抑制作用.方法:离体血管环张力试验.结果:与对照组相比,吗啡、内吗啡肽-1和内吗啡肽-2的预处理(0.1-10μmol/L)能明显降低由PE(0.1μmol/L)和Aug Ⅱ(1μmol/L)(P<0.01)诱导离体大鼠胸主动脉环的张力,但是不能减少去内皮血管的张力.纳络酮(1μmol/L)能部分阻断内吗啡肽-1和内吗啡肽-2的抑制作用(P<0.01),N^(ω)-nitro-L-arginine(10μmol/L)或血管环去内皮能完全阻断这种作用(P<0.01).结论:内吗啡肽-1和内吗啡肽-2通过纳络酮敏感方式抑制由PE和AngⅡ诱导的离体大鼠胸主动脉环收缩,这种抑制作用可能与血管内皮NO的释放有关.

关 键 词：opioid peptides ENDOMORPHINS MORPHINE PHENYLEPHRINE angiotensin Ⅱ thoracicarteries nitric oxide 

分 类 号：R96[医药卫生—药理学]