Effect of unsaturated fatty acid on muscarinic current in guinea pig gastric antral circular myocytes  

不饱和脂肪酸对豚鼠胃窦环行肌细胞毒蕈碱电流的影响(英文)

作　　者：崔艺峰[1] 金海[2] 郭慧淑[1] 李林[1] 禹永春[1] 许文燮[1] 

机构地区：[1]延边大学医学院生理学教研室 [2]延边大学医院附属医院

出　　处：《Acta Pharmacologica Sinica》2003年第3期93-98,共6页中国药理学报（英文版）

摘　　要：AIM: To investigate the effect of ectogenesis unsaturated fatty acid on carbachol-induced muscarinic current(I_CCh) and its mechanism. METHODS: Using the whole-cell patch-clamp technique, I_CCh was recorded in single smooth muscle cell isolated from the antral circular smooth mucles of guinea-pig stomach. RESULTS: Arachidonic acid （AA） was added in external perfusing solution and AA inhibited I_CCh to 46%±8%, 23%±5%, and 3.8%±0.9% at 1, 3, and 5 μmol/L. Another unsaturated fatty acid, linoleic acid（LA） also inhibited I_CCh in a dose-dependant manner. LA inhibited I_CCh to 69%±10%, 35%±5%, and 7.4%±1.2% at 1, 5, and 10 μmol/L, respectively. The same concentration （5μmol/L） of AA, LA, and oleic acid （OA） suppressed I_CCh to 3.8%±0.9%, 35%±5%, and 67%±9%, respectively. The inhibitory potency sequence of these unsaturated fatty acids was AA】LA】OA. After 10-15 min of pretreatment with H-7 （a protein phosphorylation C inhibitor） 100 μmol/L or indomethacin（a cyclooxygenase inhibitor） 10 μmol/L, I_CCh was inhibited by 5 μmol/L of AA to 5.5%±0.7% and 3.0%±1.0%, respectively. CONCLUSION: The unsaturated fatty acids directly inhibited I_CCh, and the inhibitory potency was related to the number of double bonds in fatty acid chain.目的：研究外源性不饱和脂肪酸对豚鼠胃窦平滑肌细胞素毒蕈碱电流的影响及其作用机制。方法：利用膜片箝技术的全细胞记录法在急性分离的胃窦环行肌细胞上记录毒蕈碱电流。结果：在细胞外灌流液中给予花生四烯酸（arachidonic acid,AA）明显抑制I_cch,并具有量效关系：当AA的浓度在1。3和μmo1/L时，分别抑制I_cch至46%±8%,23%±5%和3.8%±0.9%;另一种不饱和脂肪酸，亚麻酸（linoleic acid,LA）也抑制I_cch，在1,5和10μmo1/L浓度分别抑制I_cch至3.8%±0.9%,35%±5%和67%±9%；用H-7（蛋白激酶C抑制剂）100μmo1/L预处理10-15分钟以后，AA分别抑制I_cch至5.5%±0.7%和3.0%±1.0%。结论：不饱和脂肪酸直抑制毒蕈碱电流，且抑制程度与不饱和脂肪酸链中的双键数目有关。

关 键 词：unsaturated FATS MUSCARINIC receptors CARBACHOL pyloric ANTRUM GUINEA pigs 

分 类 号：R9[医药卫生—药学]