人参多糖及IL-2对小鼠黑色素瘤细胞体内的抑制效应  

INHIBITORY EFFECT OF GINSENG POLYSACCHARIDE AND IL-2 ON MOUSE MELANOMA in vivo

作　　者：谢遵江[1] 刘文庆[2] 贺业春[1] 刘颖[1] 张湛波[1] 

机构地区：[1]哈尔滨医科大学解剖学教研室,哈尔滨150086 [2]齐齐哈尔医学院解剖学教研室,齐齐哈尔161042

出　　处：《解剖学报》2002年第5期538-541,共4页Acta Anatomica Sinica

基　　金：黑龙江省教委科研基金资助项目 (980 0 0 12 )

摘　　要：目的　探讨依赖人参多糖及IL 2的肿瘤浸润淋巴细胞 (tumorinfiltratinglymphocyte ,TIL)在体内抗肿瘤作用。　方法　建立B1 6 黑色素瘤小鼠模型 ,将 30只C57小鼠随机分为 4组 ,1组瘤体周围皮下注射IL 2 ,2组注射IL 2和人参多糖 ,3组注射人参多糖 ,4组仅注射生理盐水作为对照组 ,通过测量瘤重、瘤体积和抑瘤率观察其抗肿瘤作用。同时取瘤周部位皮肤组织 ,光镜和电镜观察TIL细胞的形态和分布特征。　结果　人参多糖与IL 2联合体内应用抗肿瘤作用显著强于对照组 (P <0 0 5 )。实验组瘤周区可见不同程度的淋巴细胞浸润 ,其中以 2组为多 ,且可见TIL细胞与肿瘤细胞有膜接触。　结论　人参多糖与IL 2联合体内应用抗肿瘤作用显著强于对照组 。Objective To study on anti tumor effect of ginseng polysaccharide(GP)and IL 2 on tumor infiltrating lymphocytes(TIL) in vivo. Methods The B 16 melanoma mouse model was established.30 C 57 mice were randomly divided into 4 groups,1 group,2 group,3 group were subcutanously injected into the mouse tumor surrounding tissue with IL 2,IL 2 and GP,GP alone respectively,and the 4th group injected with NS was used as the control.After treatment,the changes of the tumor were detected.The morphological and distributive characteristics of the TIL in tumor surrounding tissue in C 57 mice were observed by light and electron microscopy. Results GP and IL 2 injected together in vivo showed a singificantly anti tumor effect which was much higher than that of the control( P <0 05).Some TIL appeared in tumor surrounding tissue,and more TIL in 2 group was seen.The membrane contacts between the TIL and tumor cell could also be observed.Conclusion\ In vivo GP and IL 2 combination treatment with more TIL was more effective than that of the GP group and IL 2 group.

关 键 词：IL-2 小鼠 人参多糖 B16黑色素瘤 肿瘤浸润淋巴细胞 体内抑制 

分 类 号：R730.5[医药卫生—肿瘤]