rhbFGF、rhTGF-β、rhBMP-2对骨髓基质细胞体外培养增殖的影响  

The effects of basic fibroblast growth factor, transforming growth factor-β1 and bone morphogenetic protein-2 on the proliferation of rat bone marrow stromal cells

作　　者：李才良 刘宏伟 任肖华 涂晓丽 

机构地区：[1]广东广州第一军医大学第一附属医院口腔科,510515

出　　处：《实用口腔医学杂志》2002年第5期456-459,共4页Journal of Practical Stomatology

基　　金：全军"十五"医药卫生科研基金指导性课题(编号 0 1L 0 75)资助课题;广东省科技计划重点攻关项目(编号C30 70 4 )资助课题

摘　　要：目的 :探讨重组人骨形成蛋白 (rhBMP 2 )、重组人转化生长因子 beta(rhTGF β1)、重组人碱性成纤维细胞生长因子 (rhbFGF)单独或联合应用对骨髓基质细胞体外培养生物特性的影响。方法 :体外培养大鼠骨髓基质细胞 ,分别用特定浓度的rhBMP 2 (2 0 0 μg/L ,简称b )、rhTGF β1(5 μg/L ,简称t)和rhbFGF(1μg/L ,简称f)单独或联合作用 ,观察它们对骨髓基质细胞增殖的影响 ,进行细胞计数测定细胞生长曲线 ,计算细胞倍增时间 ,用四唑盐比色法 (MTT)法测定细胞增殖情况。结果 :细胞计数测定显示所有各组在第 3天细胞数增加 ,对照组、t组、f组、f +t组和f +t +b组第 4天进入平顶期 ,而b组和f +b组细胞仍保持较高的增殖活性 ,细胞倍增时间f +b组最短为 3 2h ,MTT法测定结果与细胞计数法相似 ,b组和f +b组与对照组相比细胞增殖有显著差异 (P <0 .0 5 )。结论 :生长因子rhbFGF(1μg/L)和rhBMP 2 (2 0 0 μg/L)结合使用可以促进骨髓基质细胞的增殖 。Objective: To study the effects of human recombinant basic fibroblast growth factor(rhbFGF), recombinant human transforming growth factor β1 (rhTGF β1)and recombinant human bone morphogenetic protein 2(rhBMP 2) alone or in combination on the proliferation of rat bone marrow stromal cells(BMSCs) in vitro . Methods: Rat BMSCs were cultured in vitro , rhbFGF(1 μg/L,f), rhTGF β1(5 μg/L, t) and rhBMP 2(200 μg/L,b) alone or in combination were added into the culture medium, cells were examined by phase contrast microscope, cell growth curves were obtained by cell counting and cell proliferation was detected by MTT assay. Results: 1 μg/L of rhbFGF alone or combined with 200 μg/L of rhBMP 2 stimulated BMSC proliferation( P <0.05). The combination was more effective. Conclusion: rhbFGF(1 μg/L) combined with rhBMP 2(200 μg/L) may effectively stimulate BMSC to proliferate.

关 键 词：RHBFGF rhTGF-β RHBMP-2 骨髓基质细胞 体外培养 细胞增殖 

分 类 号：R780.2[医药卫生—口腔医学]