一种简单、快速筛选水稻种胚脂氧合酶-3缺失体的新方法  

One Simple and Rapid Spectrophotometric Method for Screening Lipoxygenase-3-null in Rice Embryos

作　　者：沈文飚[1] 周彤[1] 王益华[1] 俞伟伟[1] 郑天清[1] 翟虎渠[2] 万建民[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏省植物基因工程研究中心,南京210095 [2]中国农业科学院,北京100081

出　　处：《生物化学与生物物理进展》2002年第5期824-826,共3页Progress In Biochemistry and Biophysics

基　　金：科技部转基因专项资助项目 (J0 0 A0 0 6 1)~~

摘　　要：根据水稻种胚脂氧合酶 3(LOX 3)催化产物的氧化特性 ,结合脂氧合酶的专一性抑制剂———去甲二氢愈创木酸 ,建立了筛选水稻种胚脂氧合酶 3缺失体的I2 KI比色法 ,并探讨其最佳实验条件 .与常用的单克隆抗体筛选技术相比 ,该方法具有准确、简单且成本低的特点 。One rapid and inexpensive spectrophotometric method was developed to screen rice embryos for lipoxygenase-3-null, based on the oxidative ability of 9-hydroperoxide, the catalytic product of lipoxygenase-3, combined with the specific inhibitor of lipoxygenase, namely nordihydroguaiaretic acid, when purified lipoxygenase-3 or the surface of cut rice single embryo was exposed to emulsions containing linoleate. The 9-hydroperoxides were detected by one color test based on the oxidation of iodide-starch. The optimal experimental conditions for above method had also been surveyed. Compared with the normal monoclonal antibodies method, this new method is more reliable, simple, costs less reagent, which could also be used as the useful toots for breeding storable rice.

关 键 词：快速筛选 水稻种胚 脂氧合酶-3缺失体 耐储性 

分 类 号：S511.03[农业科学—作物学]