大豆异黄酮抑制人乳腺癌细胞生长及作用机制研究  

Effect of isoflavone on human breast cancer cell line MCF-7 and its probable mechanism

作　　者：方庆伟[1] 刘颖[1] 宋丹凤[1] 崔洪斌[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院,哈尔滨150001

出　　处：《卫生研究》2002年第5期367-369,共3页Journal of Hygiene Research

基　　金：国家自然科学基金 (No .30 10 0 151)

摘　　要：采用体外细胞培养的方法 ,探讨大豆异黄酮对体外培养的人乳腺癌MCF - 7细胞生长的作用及作用机制。结果表明 ,大豆异黄酮抑制MCF - 7细胞生长 ,诱导MCF - 7细胞凋亡。蛋白水平检测表明大豆异黄酮可使MCF - 7细胞iNOS表达升高。结果提示大豆异黄酮抑制MCF - 7细胞生长 ,诱导细胞凋亡 。The effects of isoflavone on human breast cancer cell line MCF-7 growth and apoptosis and its probable mechanisms were studied in vitro. The results showed that isoflavone could significantly inhibit the growth and induce the apoptosis of MCF-7 cells. Western Blotting showed that isoflavone increased iNOS protein expression. These results suggested that isoflavone significantly inhibited the MCF-7 growth and induced cell apoptosis by regulating iNOS gene expression.

关 键 词：乳腺癌 大豆异黄酮 乳腺癌细胞株 细胞凋亡 基因表达 

分 类 号：R737.9[医药卫生—肿瘤]