反相高效液相色谱法同时测定膳食补充剂中维生素C、叶酸和泛酸的方法研究  

Simultaneous Determination of Vitamin C, Folic Acid, Pantothenic Acid in Dietary Supplements with Reversed Phase High Performance Liquid Chromatography

作　　者：许雁平[1] 黄淑云[1] 

机构地区：[1]安徽省卫生防疫站,安徽合肥230061

出　　处：《安徽预防医学杂志》2002年第6期336-338,共3页Anhui Journal of Preventive Medicine

摘　　要：采用反相高效液相色谱法 ,对膳食补充剂中的维生素C、叶酸、泛酸的同时测定进行了方法学研究。色谱参考条件为SupelcosilC18柱 ;柱温 :4 0℃ ;检测波长 :2 10nm ;流动相 :0 .0 5mol/LKH2 PO4∶CH3 OH∶0 .1mol/LKOH =85 +8+7(pH6 .3) ;流速 :1.5ml/min。用外标法定量 ,线性范围为维生素C :0～ 1.0mg/ml;叶酸 :0～ 0 .1mg/ml;泛酸 :0～ 0 .2 5mg/ml。线性相关系数为 0 .999到 0 .9997,平均回收率为 90 .5 %～ 10 5 .6 % ,相对标准偏差为 1.6 %～ 8.0 %。方法简便 ,快速、准确 ,用于实际样品测定 ,结果令人较为满意。A reversed phase high performance liquid chromatography for the simultaneous determination of vitamin C. folic acid, pantothenic acid in dietary supplements was developed. The optimized chromatographic conditions were: Supelcosil C 18 column, column temperature: 40℃, detection wavelength: 210nm, mobile phase: 0.05mol/L KH 2 PO 4 :CH 3 OH:0.1mol/L KOH=85+8+7(pH6.3), flow rate: 1.5ml/min.The quantitative analysis of the three vitamins was achieved with external standard method . The linear range was 0～1.0mg/ml for ascorbic acid , 0～0.1mg/ml for folic acid and 0～0.25mg/ml for pantothenic acid. The correlation coefficients ranged from 0.999 to 0.9997. The average recovery of the method was 90.5%～105.6%. The relative standard deviation was 1.6%～8.0%. The method was simple, rapid and accurate. It was applied for detecting actual samples with satisfactory result.

关 键 词：反相高效液相色谱法 膳食补充剂 维生素C 

分 类 号：R151.2[医药卫生—营养与食品卫生学]