HER2/neu诱导DBA/2小鼠特异性CTL应答及其抑瘤效应研究  

Induction of specific CTLs and anti-tumor effect in DBA/2 mice by HER2/neu genetic immunization

作　　者：方路[1] 吴玉章[1] 

机构地区：[1]第三军医大学基础医学部全军免疫学研究所,重庆400038

出　　处：《第三军医大学学报》2002年第10期1137-1139,共3页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划资助项目 ("973"项目 ) ( 2 0 0 1CB510 0 0 1)

摘　　要：目的　探讨HER2 neu原癌基因DNA疫苗免疫诱导特异性细胞免疫应答及其在体内的抑瘤效应。方法　将重组HER2 neu原癌基因真核表达载体转染P815细胞 ,用细胞组织化学及Western blot方法鉴定质粒在细胞中的表达。在DBA 2小鼠体内构建表达HER2抗原的肿瘤动物模型 ,通过重组质粒免疫小鼠 ,诱导小鼠产生细胞免疫应答 ,观察免疫动物体内的抗瘤效应。结果　在体外获得了稳定表达HER2 neu基因的P815细胞 ;免疫小鼠脾细胞中诱导出特异性CTL ,并杀伤HER2 neu阳性的P815细胞 ;荷瘤小鼠的肿瘤生长受抑 ,存活期延长。结论　HER2 neu原癌基因DNA疫苗免疫可诱导特异性细胞免疫应答 ,并具有一定抗瘤效应。Objective To study the cellular immune response and the anti tumor effects induced by intramuscular DNA immunization with HER2/neu gene. Methods HER2/neu oncogene vector was transfected into P815 cells, and the expression of the gene DNA was identified with immunocytochemistry and Western blotting. The cellular immune response and anti tumor effects of the transfectants were analyzed by examinating the expression of HER2 in DBA/2 mice after intramuscular DNA immunization. Results P815 cells were observed to express HER2/neu oncogene in vitro . HER2/neu specific CTLs were induced in immunized DBA/2 mice, which showed specifically killing effects on HER2/neu positive P815 cells. Tumor growth was inhibited and the survival of mice was prolonged. Conclusion Specific cellular immune response and anti tumor effect are elicited by HER2/neu gene immunization.

关 键 词：HER2/NEU基因 细胞免疫 抗瘤效应 肿瘤治疗 靶抗原 DNA疫苗 

分 类 号：R730.51[医药卫生—肿瘤] R392[医药卫生—临床医学]