酶促合成肽的研究 Ⅱ.含羧基侧链氨基酸的肽的酶促合成  

Studies on Enzymic Synthesis of Oligopeptides II. Thermolysin-Catalyzed Synthesis of Peptides Containing a Side Chain Free Carboxyl Group

作　　者：汪猷[1] 冯冬梅[1] 徐杰诚[1] 陈玲玲[1] 

机构地区：[1]中国科学院上海有机化学研究所

出　　处：《化学学报》1989年第3期266-269,共4页Acta Chimica Sinica

摘　　要：用嗜热菌酶酶促合成了一系列含门冬氨酸、谷氨酸的肽。实验证明,缩合反应中羧基组份中的门冬氨酸β-羧基或谷氨酸γ-羧基毋需保护。同时也研究了邻近氨基酸残基对缩合反应的影响。A number of peptides were synthesized from the carboxyl component containing a free side chain carboxyl group of aspartyl or glutamyl residue through thermolysin catalysis. The effects of neighboring residues situated closely to a C-terminal glutamic or aspartic acid in the carboxyl component on the formation of peptide bond were studied. The efficiency of coupling by thermolysin varied with the nature of the amino component and the carboxyl component. Strict sbereospecificity was observed.

关 键 词：肽 酶促合成 羧基侧链 氨基酸 

分 类 号：O629.72[理学—有机化学]