血清胸腺因子(STF)基因的合成  

CHEMICAL SYNTHESIS OF A GENE FOR SERUM THYMIC FACTOR

作　　者：温晋[1] 陈亚民[1] 梁建宁 周金鑫[1] 刘飞鹏[1] 周天鸿[1] 

机构地区：[1]暨南大学生物学系

出　　处：《暨南大学学报（自然科学与医学版）》1991年第3期84-87,共4页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省科委资助课题

摘　　要：利用381A型DNA合成仪,合成了用于编码血清胸腺因子(STF)基因的DNA片段。该片段两端带有两个不同限制性内切酶(Sal I,BamHI)识别位点的粘性末端,中部则为以起始密码子ATG开头并以终止密码子TAG及TAA结尾的多肽(STF)编码序列。将合成STF基因克隆到单链噬菌体M13作双脱氧法定序,定序结果表明合成基因与设计预期结果相符。A chemically synthesized DNA fragment encoding for serum thymic factor (STF) was achieved by using Mqdel 381A DNA synthesizer. The synthetic fragment was designed in such a way that the coding sequence of STF gene is preceeded by ATG for the starting of translation and followed by ATG and TAA for the stopping. The ends of the fragment are BamHI and Sall recogartion Sequence separatly. The synthetic STF gene which cloned in the singlestranded phage M13mp18 was analysed by the method of dedeoxy chaintermination DNA sequencing. The sequence determined demonstrated that the synthetic STF gene was exactly the same as designed.

关 键 词：血清胸腺因子 基因 合成 DNA片段 

分 类 号：Q781[生物学—分子生物学]