利用PCR技术鉴别普通小麦Glu-1位点的某些等位基因  

Identification of Some Alleles at Glu-1 Loci in Common Wheat with PCR Approach

作　　者：孙辉[1] 刘志勇[1] 李保云[1] 刘广田[1] 

机构地区：[1]中国农业大学作物遗传育种系

出　　处：《作物学报》2002年第6期734-737,共4页Acta Agronomica Sinica

基　　金：国家自然科学基金资助项目 ( 39370 440 )

摘　　要：Glu- 1位点的等位基因变异与普通小麦的烘烤品质有密切的关系 ,本文利用根据 Glu- Dly位点的基因序列设计的一对引物 ,通过 PCR技术 ,可以准确鉴别等位基因的变异 Glu- Dly10和 Glu- Dly12 ,同时还可以初步鉴定 Glu- B1位点的等位基因变异 Glu- Blh(14+15 ) 。PCR technich is used to detect allelic variation at Glu-1 loci in common wheat. With a pair of synthetic primers, specific variation for Glu-Dly, Glu-Dly 10 and Glu-Dly 12 can be distinguished clearly. Besides, Glu-B1h (subunit pair 14+15) can also be identified with the same primers. Compared with SDS-PAGE, PCR approach provides a more rapid and efficient detection of some alleles at Glu-1 loci in the early generation of wheat breeding program.

关 键 词：PCR技术 鉴别 等位基因 普通小麦 GLU-1位点 烘烤品质 

分 类 号：S512.1[农业科学—作物学]