孕鼠补充苏氨酸锌对仔鼠生长及其相关基因表达的影响  

Effect of Prepartum Supplementation of Pregnant Rats with Zinc Threoninate on the Growth Performance and Related Genes Expression of Their Offspring

作　　者：虞俊翔 冯建萍[1] 谢明勇[1] 王光然[1] 周寰[1] 胡晓波[1] YU Junxiang;FENG Jianping;XIE Mingyong;WANG Guangran;ZHOU Huan;HU Xiaobo(State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China)

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《食品科学》2017年第5期239-243,共5页Food Science

基　　金：江西省科技支撑计划项目(20151BBF60040);江西省自然科学基金项目(20132BAB204002);江西省重大科技创新研究项目(20124ACF00400);江西省教育厅2011年度产学研合作项目(GJJ11002)

摘　　要：为研究母体补充苏氨酸锌对子代生长水平的影响,实验将40只受孕SD大鼠随机分为5组,于孕鼠妊娠期第6~15天分别灌胃补充高、中、低剂量苏氨酸锌(灌胃剂量分别为250、500、1 000 mg/(kg·d)),孕鼠分娩后将仔鼠常规饲养,并测量1月龄仔鼠的生长指标和相关基因的表达。结果表明,苏氨酸锌各剂量组仔鼠体质量与对照组相比有显著提高(P<0.05);苏氨酸锌各剂量组与对照组相比较,其组织中锌转运体Zn T1基因的相对表达量都有显著提高(P<0.05);苏氨酸锌高剂量组与对照组相比较,其组织中胰岛素样生长因子IGF1基因相对表达量都有显著提高(P<0.05);且胰岛素样生长因子受体基因IGF1R的表达与IGF1的表达趋势基本保持一致。实验说明妊娠期补充苏氨酸锌能在一定程度上提升仔鼠的生长性能,能显著提高仔鼠锌转运体Zn T1、胰岛素样生长因子IGF1及其受体IGF1R基因表达水平。The purpose of this study was to observe the effect of prepartum supplementation of zinc threoninate(Thr-Zn)for pregnant rats on the growth performance of their offspring.Forty pregnant SD rats were randomly divided into five groups.Thr-Zn at high,medium and low doses during the sixth to fifteenth day of pregnancy was orally administered to the three experimental groups of animals,respectively.After the newborn rats were routinely fed to one month of age,the growth indicators and the expression of growth-related genes were evaluated.Compared with the control group,body weights of the offspring rats from each dose group increased significantly(P<0.05).In addition,the mRNA expression levels ofzinc transporter1(ZnT1)and insulin-like growth factor1(IGF1)were increased significantly(P<0.05).The expression of insulin-like growth factor receptor(IGF1R)exhibited the same trend as IGF1.Therefore,the growth performance of offspring rats could be improved,and the mRNA expression levels of ZnT1,IGF1and IGF1R could be increased significantly by prepartum zinc supplementation.

关 键 词：苏氨酸锌 锌转运体ZnT1 胰岛素样生长因子IGF1 胰岛素样生长因子受体 基因相对表达量 

分 类 号：TS201.6[轻工技术与工程—食品科学]