慢性功能性便秘患者的肠道菌群分析  

Structure analysis of the gut microbiota in chronic functional constipation patients

作　　者：黄林生[1,2,3] 高仁元[2,3] 严雪冰 祝琦[2,3] 潘成[2,3] 尹明明[2,3] 李豪[2,3] 孔程[2,3] 张鹏[2,3] 沈通一[2,3] 秦环龙[1,2,3] Huang Linsheng;Gao Renyuan;Yan Xuebing;Zhu Qi;Pan Cheng;Yin Mingming;Li Hao;Kong Cheng;Zhang Peng;Shen Tongyi;Qin Huanlong(Shanghai Tenth People′s Hospital Clinical College of Anhui Medical University;Department of General Surgery, Shanghai Tenth People′s Hospital, Tongji University School of Medicine;Research Institute for Intestinal Diseases of Tongji University School of Medicine,Shanghai 200072, China)

机构地区：[1]安徽医科大学上海十院临床学院,上海200072 [2]同济大学附属第十人民医院普外科,上海200072 [3]同济大学医学院肠道疾病研究所,上海200072

出　　处：《中华结直肠疾病电子杂志》2017年第2期121-126,共6页Chinese Journal of Colorectal Diseases(Electronic Edition)

基　　金：国家自然科学基金项目(No.81230057;No.81472262;No.81302066);上海新兴前沿技术联合攻关项目(No.SHDC12012106)

摘　　要：目的探讨慢性功能性便秘患者与健康对照组之间肠道菌群的差异。方法选取便秘组和健康对照组共计20人,留取新鲜粪便样本,冰块运送至实验室并存放于-80度冰箱。采用16S r RNA测序技术检测V4区鉴定菌群。结果发现两组人群的菌群丰富度差异有统计学意义(P<0.05),菌群多样性差异无统计学意义。便秘患者与健康对照组之间的肠道细菌组成在门和属水平差异均存在显著统计学意义(P<0.05)。在门水平,便秘患者肠道中放线菌门(Actinobacteria)丰度显著增加(Z=-3.10,P<0.05),而变形菌门(Proteobacteria)丰度显著降低(Z=-2.42,P<0.05),在属水平发现20个菌属差异存在统计学意义(P<0.05)。其中,Coprobacillus(Z=-2.10,P<0.05)、Hungatella(Z=-2.31,P<0.05)、Holdemanella(Z=-2.32,P<0.05)、Anaerostipes(Z=-3.78,P<0.01)、Bacteroidales_S24-7_group_norank(Z=-2.15,P<0.05)、Bacteroides(Z=-3.78,P<0.01)、Blautia(Z=-3.78,P<0.05)、Collinsella(Z=-3.02,P<0.05)、Dorea(Z=-2.95,P<0.05)、[Eubacterium]_ruminantium_group(Z=-2.70,P<0.05)、Erysipelotrichaceae_UCG-003(Z=-3.10,P<0.05)、Ruminococcaceae_uncultured(Z=-2.50,P<0.05)、Parabacteroides(Z=-2.87,P<0.05)菌属在便秘患者的肠道中丰度明显增多,而Megasphaera(Z=-2.31,P<0.05)、Paraprevotella(Z=-2.94,P<0.05)、Prevotella_2(Z=-3.10,P<0.05)、Prevotella_9(Z=-3.68,P<0.01)、Enterococcus(Z=-2.27,P<0.05)、Catenibacterium(Z=-2.48,P<0.05)、Clostridium_sensu_stricto_1(Z=-2.50,P<0.05)菌属在健康对照组的肠道中丰度明显增多。结论慢性功能性便秘患者的肠道菌群与健康人群的菌群存在较大差异,通过改变肠道菌群可能成为治疗慢性功能性便秘的新策略。Objective To investigate the differences of gut microbiota between patients withchronic functional constipation(CFC)and health controls(HC).Methods16S rRNA highthroughoutpyrosequencing on V4region was employed to examine the difference of gut microbiota profiles between10CFC patients and10HCs.The obtained data were analyzed using bioinformatics.Results The richnessanalysis suggested that HCs have richer gut microbiota than CFC patients(all P<0.05),while no significantdifference was observed in the diversity analysis.There was a significant structure difference of gut microbiotabetween CFC patients and HCs both at the phylum and genus levels(all P<0.05).At the phylum level,Actinobacteria in CFC patients is richer than that in HCs,but Proteobacteria is opposite(all P<0.05).At thegenus level,20gut microbiota were found to have a changed relative abundance between CFC patients andHCs,in which Coprobacillus,Hungatella,Holdemanella,Anaerostipes,Bacteroidales_S24-7_group_norank,Bacteroides,Blautia,[Eubacterium]_ruminantium_group,Collinsella,Dorea,Erysipelotrichaceae_UCG-003,Parabacteroides and Ruminococcaceae_uncultured were increased in CFC patients while Megasphaera,Paraprevotella,Prevotella_2,Prevotella_9,Enterococcus,Catenibacterium and Clostridium_sensu_stricto_1wereincreased in HCs.Conclusion There was a significant structure difference of gut microbiota between CFCpatients and HCs.Therefore,targeting the gut microbiota dysbiosis may be a promising therapeutic strategyfor CFC patients.

关 键 词：便秘 肠道菌群失调 菌群丰度 16S RRNA 

分 类 号：R574.62[医药卫生—消化系统]