耐盐自养铁基反硝化菌的分离鉴定及特性研究  

Isolation, Identification and Characteristics of a Salt-Tolerant Autotrophic Ferrous-Dependent Denitrifying Bacterium

作　　者：王茹[1] 叶贞[1] 黄伟辰 许少怡 戴陈琳 梁璐怡[1] 郑平[1] WANG Ru;YE Zhen;HUANG Wei-chen;XU Shao-yi;DAI Chen-lin;LIANG Lu-yi;ZHENG Ping(Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China)

机构地区：[1]浙江大学环境工程系,浙江杭州310058

出　　处：《高校化学工程学报》2017年第2期456-462,共7页Journal of Chemical Engineering of Chinese Universities

基　　金：国家自然科学基金(51278457;51578484);国家青年基金(51608474);浙江省创新团队(2013TD12)

摘　　要：针对高盐、低C/N比废水脱氮难题,从实验室稳定运行的高效铁盐脱氮反应器中分离获得了一株耐盐自养反硝化菌株,命名为NF-5。形态观察表明,菌株NF-5个体呈杆状,大小(0.80~1.2)μm×(1.7~2.3)μm,革兰氏阳性;经生理生化试验和16S rRNA测序测定分析,菌株NF-5可归入Isoptericola属。菌株NF-5能够利用亚铁作为电子供体,利用硝酸盐作为电子受体。当起始硝氮浓度为50 mg·L^(-1)(铁氮比为5:1),菌株接种量OD_(600)为0.52(稀释18倍)时,7 d后反应体系中硝氮去除率为82%,硝氮去除速率高达0.31 mg N·(L·h)^(-1)。菌株NF-5耐盐性能良好,可耐受范围为10~50 g·NaCl·L^(-1),最适盐度为20 g·NaCl·L^(-1)。当盐浓度为50 g·NaCl·L^(-1)时,菌悬液中活细胞比例为54%,反应体系中反硝化活性仅下降47%。In order to improve nitrogen removal from salinity wastewater with low C/N,a salt-tolerant autotrophic ferrous-dependent denitrifying bacterium(NF-5)was isolated from a high-efficient lab-scale ferrous-dependent denitrifying reactor.NF-5is in rod shape and Gram positive with a size of(0.80~1.2)μm×(1.7~2.3)μm.It can be classified as genus Isoptericola based on bio-chemical characteristics and16S rRNA sequence analysis.NF-5can use nitrate as electron accepter and ferrous as electron donor.When the initialNO3-N concentration is50mgL1(Fe/N value=5:1)and OD600=0.52(after dilution of18times),nitrate emoval ratio of NF-5in the reaction system is82%after one week and the maximum denitrifying rate is0.31mg N(Lh)1.NF-5shows impressive salt-tolerance in the range of10~50g NaClL1,and the optimal salt concentration is20g NaClL1.54%of total bacteria in suspension keeps alive even under50g NaClL1,and the denitrifying activity only decreases by47%.

关 键 词：耐盐铁基反硝化菌 分离鉴定 脱氮特性 耐盐特性 

分 类 号：X172[环境科学与工程—环境科学]