长果颈黄芪根中抑制α-葡萄糖苷酶活性成分研究  

Study on the Compounds with α-Glucosidase Inhibitory Activity from Roots of Astragalus englerianus

作　　者：邱琳 肖朝江 董相 姜北 Qiu Lin;Xiao Chaojiang;Dong Xiang;Jiang Bei(Institute of Materia Medica of Dali University, Dali, Yunnan 671000, China;College of Pharmacy and Chemistry,Dali University, Dali, Yunnan 671000, China)

机构地区：[1]大理大学药物研究所,云南大理671000 [2]大理大学药学与化学学院,云南大理671000

出　　处：《大理大学学报》2017年第4期1-5,共5页Journal of Dali University

基　　金：国家自然科学基金资助项目(31170313)

摘　　要：目的:对长果颈黄芪(Astragalus englerianus)根化学成分进行研究,并对分离得到的化合物进行α-葡萄糖苷酶抑制活性测试。方法:利用Rp-18、Sephadex LH-20、硅胶等分离手段对长果颈黄芪根部分的甲醇提取物进行分离纯化,通过现代波谱学技术对相关化合物进行结构鉴定,并以4-硝基酚-α-D-吡喃葡萄糖苷(PNPG)为底物的酶抑制剂筛选模型对各样品进行α-葡萄糖苷酶抑制活性实验。结果:从该植物根甲醇提取物的乙酸乙酯部位分离得到6个化合物,分别鉴定为:十六烷酸单甘油酯(1)、2,4'-二羟基-4-甲氧基二氢查耳酮(2)、胡萝卜苷(3)、5α,6β-二羟基胡萝卜苷(4)、(3R)-vestitol-7-O-glucoside(5)、(2S,3S,4R,8E)-2-[(2'R)-2'-羟基-二十四烷酰胺]-8-十八烯-1,3,4-三醇(6)。α-葡萄糖苷酶抑制活性结果显示,化合物2具有显著抑制活性,IC50显著小于阳性对照阿卡波糖;化合物5及其苷元(3R)-vestitol也显示一定的抑制活性。结论:化合物1~6均为首次从长果颈黄芪根中分离得到,其中黄酮类成分对α-葡萄糖苷酶具有较好的抑制活性。Objective:To study the chemical components of Astragalus englerianus,and to explore the active compounds ofα-glucosidase's inhibiting from the roots of Astragalus englerianus.Methods:The compounds were isolated and purified by Rp-18,Sephadex LH-20and silica gel column chromatography.Their structures were identified by modern spectroscopy techniques.Theα-glucosidase's inhibiting experiment was performed by using enzyme inhibitor screening model with4-nitrophenolα-D-glucopyranoside(PNPG)as the substrate.Results:Six compounds were isolated from ethyl acetate fraction of methanol extract of the roots of A.englerianus and identified as1-O-hexadecanolenin(1),2,4'-dihydroxy-4-methoxy dihydrochalcone(2),daucosterol(3),5α,6β-dihydroxy-daucosterol(4),(3R)-vestitol-7-O-glucoside(5),and(2S,3S,4R,8E)-2-[(2'R)-2-hydroxytetracosanoylamino]-1,3,4-octadecanetriol-8-ene(6).All of these compounds were examined for theirα-glucosidase inhibitory activities.As a result,compound2showed significantly activity with the lower IC50than the positive control,acarbose.Compound5and its aglucon,(3R)-vestitol also showed some inhibitory activity.Conclusion:All of the compounds were isolated from the roots of A.englerianus for the first time,and the flavonoids showed the betterα-glucosidase inhibitory activity than the other compounds.

关 键 词：长果颈黄芪 根 化学成分 Α-葡萄糖苷酶 抑制活性 

分 类 号：R932[医药卫生—生药学]