植物乳杆菌天然质粒分类  

Classification of Natural Plasmids in Lactobacillus plantarum

作　　者：孙大庆[1] 李洪飞[1] 宋大巍[2] 杨建[1,2] SUN Daqing;LI Hongfei;SONG Dawei;YANG Jian(National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, China;College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China)

机构地区：[1]黑龙江八一农垦大学国家杂粮工程技术研究中心,黑龙江大庆163319 [2]黑龙江八一农垦大学食品学院,黑龙江大庆163319

出　　处：《食品科学》2017年第12期69-74,共6页Food Science

基　　金：黑龙江省青年科学基金项目(QC2014C020)

摘　　要：为建立植物乳杆菌天然质粒分类方法,以质粒复制起始蛋白(replication initiation protein,Rep)作为分类标记,通过系统进化树分析方法,将植物乳杆菌53个编码Rep天然质粒划分为6个质粒类型,包括5个质粒家族和1个新的复制类型质粒,之后进一步提出了每个质粒家族特有的骨干序列,最终建立了一种简单、有效的植物乳杆菌天然质粒的分类方法和标准。与以往质粒功能和不相容性分类方法相比较,本方法具有较好的实用性和通用性。This study aimed to establish a classification method for natural plasmids of Lactobacillus plantarum(L.plantarum)by using plasmid replication initiation protein(Rep)as a taxonomic marker.Fifty three plasmids encodingRep in L.plantarum were divided into six plasmid types by the method of phylogenetic analysis,including five plasmidfamilies and one novel replication type of plasmid.Then,the backbone sequence specific to each plasmid family wasfurther proposed.Finally,a simple and effective classification method and criterion for L.plantarum natural plasmids wasestablished.Compared with the previous classification methods based on plasmid function and incompatibility,this methoddisplayed relatively better practicability and versatility.

关 键 词：植物乳杆菌 质粒 基因组 复制起始蛋白 复制子 

分 类 号：Q19[生物学—普通生物学]